Dear flowers I wondered if you can help.... I want to purchase three (ideally four) highly cross adsorbed secondary antibodies recognising goat, rabbit and mouse with conjugates such that I can correlate data that I obtain on a FACS Calibur with a Zeiss axioplan microscope (mercury short arc lamp). I would like at least one of the combinations (mouse and the rabbit preferably) conjugates to FRET. Our (current) filter sets on the FACS are FL1; 530/30 BP FL2; 585/42 BP FL3; 670LP FL4; 661/16BP Our Zeiss microscope filter sets are 1. Zeiss Filter set 10 : Ex 450-490: dichroic 510 Em: 515-565 2. Zeiss Filter set 37 : Ex 450/50BP: 480FT: Em: 510/50 3. Zeiss Filter set 15 : Ex 546/12BP: 580FT: Em: 590LP I could buy another one/(two) filter sets (to give us four colours) to give us channels on the microscope equating to FL3 and FL4. I'm trying to figure this out myself but I could really use some expertise.... Can any of you clever people suggest some appropriate conjugate combinations or even antibodies? Many thanks Nicola Nicola J. James PhD School of Biochemistry and Microbiology, Garstang Building, University of Leeds, Leeds, LS2 9JT. W: 0113 343 3130 M: 07973 759898 Nicola J. James PhD School of Biochemistry and Microbiology, Garstang Building, University of Leeds, Leeds, LS2 9JT. W: 0113 343 3130 M: 07973 759898Received on Thu Jun 23 14:58:00 2005
This archive was generated by hypermail 2.1.8 : Sat Jan 14 2006 - 22:03:48 EST
