Hi Alexander Yes both DsRed and CFP work well in mice. I have made multicolor mice with different fluorescent proteins expressed on different cell populations (green B cells, red T cells...) For flow cytometry DsRed and GFP/YFP work well together, on a calibur the only useful channel left with two Fluorescent proteins is FL4 because DsRed comes in on both FL2 and FL3. For true multicolor you might need to get hold of a cyan or similar machine. Using customized filters on a cyan you can analyze 4 different fluorescent proteins and 3 other antibodies, using CFP, GFP, YFP and DsRed + 1 channel off 488 (PE-CY7)+ 2 channels off the HeNe laser (APC) (APC-CY7). Of course the true beauty of these mice is only seen by microscopy. I am not currently distributing any mice, however there are DsRed expressing mice available from Jackson. Cheers Mark ============================ Dr. Mark Coles Molecular Immunology National Institute for Medical Research Mill Hill, London, NW7 1AA UK Phone: UK (0) 208 816 2685 Fax: UK (0) 208 816 2248Received on Thu Apr 21 11:58:00 2005
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