RE : simple and cheap fluorescence-capable microscope

Hi Cliff,

I don't known which kind of cytometer you have, but there is a system on Becton cytometer
who is called Acccudrop. You sort APC beads (Accudrop beads) and you verify you sorting
(in real time) by illumation of your streams with a small red diode and view with a
camera and a LP 650 filter if you have beads in your sorted stream (and in your central
stream). This system work on Facs Vantage. On the Facs Aria, you this system but better,
the system, on the window of the sorted streams, give directly (in real time)the
percentage of beads in each stream. This system is really a huge saving of time versus a
manual counting.
If you have a good handyman, with small red diode and a webcam, you can do that else you
can call a Becton dealer.
Yours, sincerly.
Marc.

-----Message d'origine-----
De : Cliff McArthur [mailto:cliff@itsa.ucsf.edu] 
Envoyé : mercredi 13 avril 2005 19:50
À : Cytometry Mailing List
Objet : simple and cheap fluorescence-capable microscope

Hi All,

I've got some incredibly valuable potential solutions from Howard already 
(no surprise, of course - thanks Howard!), but I want to query the rest of 
this list too.	I've also been through the archives and found nothing since 
2001, which is a lot of information in and of itself regarding the need for 
such a device...

However, I am still looking for a simple and inexpensive fluorescence 
microscope for the purpose of determining drop delay, you know, just for 
seeing bright beads.  Unfortunately, my lab and I have moved far away from 
the microscope I've used for years.  I have no doubt you are all very 
familiar with this particular need.  I don't need to manually check delay 
often, and that's about 99% of the need for the microscope (I can walk down 
the hall if I need to see cells or anything else.)  So, I don't want to, 
and can't, spend $17,000 - $25,000 or more on a nice set-up if all I need 
to do is see beads.

Those of you who have some such simple 'scope and can share information, 
I'd very much appreciate hearing from you.  Suggestions of any type will 
bring rave Thanks!

Cliff


Cliff J McArthur, MT (ASCP)
Specialist, Flow Cytometry/High-Speed Cell Sorting
Departments of Medicine and Microbiology/Immunology
University of California at San Francisco
521 Parnassus Ave., C430
San Francisco CA 94143-0654
415-502-6860