Tru Upstate Cell Signaling Solutions (www.upstate.com), they have a polyclonal rabbit Igg which you could conjugate with the Zenons (PE, Alexa 647, or APC). It has not been tested for flow, however, only WB and IP. Joanne Lannigan, MS Director, Flow Cytometry Core Facility University of Virginia Jordan Hall, Room 7067 P.O. Box 800734 Charlottesville, VA 22908-0734 Office: 434-924-0274 Lab: 434-243-2695 Fax: 434-982-1071 email: joannelannigan@virginia.edu > -----Original Message----- > From: AlexSchmitz [mailto:axs@mb.au.dk] > Sent: Tuesday, April 05, 2005 1:57 AM > To: Cytometry Mailing List > Subject: HA antibody for Flow (not FITC) > > Hi Flowers, > I am looking for a suitable antibody that can be used to detect > our fusionprotein containing the HA sequence YPYDVPDYA > with FlowCytometry. > > The Google search pointed to: > 1) Molecular probes/ Invitrogen monoclonal anti HA mouse IgG1 - > conugated either with Alex488 (or Alexa594). > But I want to use it in combination with GFP, therfore FITC or Alexa488 > are not an option. > 2) Anti HA mouse IgG1 monoclonal unconjugated (meaning it could be > labelled with eg. the Zenon Alexa Fluor 647 mouse > IgG1 kit). It is from HyTest clone C102. (not tested for flow) > > Does anyone have experience with these antibodies (especially the latter > one) ? > Does anybody use alternative antibodies to the detection of HA ? > What about polyclonals ? > > Many thanks in advance for your help > Alexander Schmitz > > > --Received on Wed Apr 6 12:58:00 2005
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