Thanks to all who replied to my question on CFSE alternatives. Here is a summary of the suggestions. ------------------------------------------------------------------------ ---------------------------------------------------------------------- >From Mol Probes. Not as pretty as CFSE, but it works with a red diode laser. CellTrace(tm) Far Red DDAO-SE *special packaging* Catalog Number C34553 --------------------------------------------------------------- You could try SNARF from molecular probes. It emits in the FL2 or FL3 channel. ------------------------------------------------------------------------ ------------------------------------------ Ki-67 is just as good as CFSE. ------------------------------------------------------------------------ --------- In my experience, with flow-based LPAs from hiv-infected patients, CFSE provided indirect evidence of toxicity (lower proliferative activity in CFSE-stained versus non-stained samples). I opted for PKH26 from Sigma. It runs on FL2. The main problem with PKH is the price (much more expensive). It also provides less precise peaks, but use of Modfit software for analysis compensated for this problem. ------------------------------------------------------------------------ ------- PKH-26 or the other PKH dyes. These bind to lipids but can be used for the same purpose ------------------------------------------------------------------------ -------------------- PKH 26 or PKH67 are excellent alternatives...they stain the lipid membranes in stead of the intracellular proteins. We use them all the time. Roger A. Burger, Ph.D. Technical Scientist Miltenyi Biotec, Inc. 12740 Earhart Ave. Auburn, CA 95602 530-887-5351 fax: 530-745-2851Received on Fri Mar 25 14:58:00 2005
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