.And DakoCytomation sells a Cyan bead , Spectra-Comp CYAN, order#K011611 for $467 that works pretty good for us through a 470/20. Gene Manager, Flow Cytometry Facility UCONN Health Farmington, CT. 06032 http://flowcytometry.uchc.edu 860 679 7567 -----Original Message----- From: AlexSchmitz [mailto:axs@mb.au.dk] Sent: Thursday, March 10, 2005 10:49 AM To: cyto-inbox Subject: Finding the 458nm laser line of a Coherent innova Spectrum Laser70 on FACSVantageSE Hi FlowCytonauts, I ran into trouble finding the 458nm laser line of our Innova Spectrum70 Laser on our FACSVantage SE for ECFP analysis. Actually I can tune to 6 lines below the very strong 488nm line. (UV is probably not visible) The Innova handbook says: 70C-Spectrum: 488.0: 0.25Watt; 476.5: 0.10 472.2: ---- 465.8: ---- 457.9: 0.03 Multiline UV: 0.05 So which one is the 458 ?. The first visible one ? Unfortunately I currently have no strong ECFP expressing cell line for fishing in the dark. Are there any beads that are somewhat specific for that laserline ? I tried Sphero-Yellow bright with not much success... I am running the laser as secondary laser (488 primary, outsplitted UV as laser3). I used a 649LP filter and collected the reflected light with a special 473/19 filter (as discussed previously in that group). Any help is greatly appreciated ... /Alex -- --Received on Fri Mar 11 12:58:00 2005
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