Intracellular staining with QuantumDots

From: Max Warncke <maxwarncke@ema-syn.de>
Date: Fri Mar 04 2005 - 16:47:09 EST
Do I have to use a very strong permeabilization protocol if I want to stain
intracellular antigens with QDots?

I tried to stain MHCII in and on murine (not fully matured) DC and
visiualized them on the confocal. Compared to FITC I get a brighter
extracellular fluorescence with QDots (525), but with the FITC antibody I
can see a bright signal inside the cell, which is totally absent with QDots.

For permeabilization I used Triton-X, counterstained with DAPI and
Phalloidin Alx594.

Many thanks

Max
Received on Mon Mar 7 14:38:00 2005

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