Hi Joan, dsRed does have a bit of an excitation peak around 488nm and can be excited with the usual blue laser. We have had great success with it *once the user is sure that their transfection technique works*. We have used it in combination with GFP using the standard FITC and PE filters (although we sometimes use a 510/10 for the GFP). Good luck! Derek >I have a client who is using GFP and BD's DsRed2. They are having >complete success with the GFP and getting nothing measurable with >the DsRed2. Although the literature that comes with the DsRed 2 >indicates that it can be excited by a 488 Argon Laser, I am >beginning to wonder. Looking at it's excitation peak, 488 is pretty >low on the shoulder. > Does anyone have experience measuring DsRed2 with a 488 >laser? If so is there any trick to it. Thanks in advance for any >and all help. > Sincerely, > Joan K -- *************************************************************** Derek Davies Voice: (44) 020 7269 3394 FACS Laboratory, FAX: (44) 020 7269 3479 London Research Institute, e_mail: derek.davies@cancer.org.uk Cancer Research UK mobile: 07790 604112 44 Lincolns Inn Fields, London, UK. Web Page: http://science.cancerresearchuk.org/sci/facs/ In tenebris lux ***************************************************************Received on Mon Feb 21 13:58:00 2005
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