Hey FLOWers, My colleague is performing a 3-day assay (Intracellular Cytokine) on the FACScan. She is unable to maintain cell viability. After Day 1 of the protocol, 70% of the cells are dying! What is she doing wrong--if anything? Could it be the way the lymphocytes are initially processed? I have attached the lab protocol. Thanks in advance. Fellow FLOWer, Israel This attachment - 'Procedure for Setting Compensation for Multi color analysis.doc' - 23.55 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/9bc9f67e5e45d84fe4d0e73e24a98e0349fb19ac.doc This attachment - 'Protocol for staining intracellular cytokine_GP100TRP2.doc' - 38.40 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/e5b210b725d2b4539d352c9d4e2c2aa10a20211a.doc This attachment - 'Protocol for staining intracellular cytokine_GP100TRP2-new.doc' - 51.71 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/2398de2ba87605d61beef019a0be35faf57cb7f6.docReceived on Fri Feb 18 16:38:00 2005
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