Hi Flowers, Sometimes it is not easy to do the hardware compensation when dealing with very small number of cells. I collect my FACS data with BDCalibur and try to do software compensation using FlowJo 6.0 on Mac (OS 9.0). I followed the manual step by step: define the matrix using single color sample--> save the matrix --> load the matrix--> apply it to my samples of interest. What troubles me is that after I saved the matrix I couldn't load it. I couldn't find its location using the search function in the computer although it appeared on the manual under COMPENSATION BAR. I wonder if it is a bug for this software (I have no problem with Window system using the demo data). Does anyone out there have some ideas? AlexReceived on Fri Feb 18 15:18:00 2005
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