RE: Surface markers for gut epithelial cells

Fred,

I already have CD45 included in the analysis, but with tissue derived single
cell suspensions I find we can clean up a lot of noise using a "dump"
channel for all the junk and autofluorescent cells. Now that we are finally
over the polychromatic hurdle, and have lots of colors to play with, I
thought why not try and do this in the best way possible. This may be of
particular advantage when looking for rare events....

Calman

> ----------
> From: 	Preffer, Frederic I., Ph.D.
> Sent: 	Wednesday, February 16, 2005 16:27
> To:	Prussin, Calman (NIH/NIAID); Cytometry Mailing List
> Subject:	RE: Surface markers for gut epithelial cells
> 
> Dear Calman
> 
> I have had mixed results with surface -available epithelial antigens. Any
> reason
> why just CD45+ won't help??
> 
> Fred
> 
> -----Original Message-----
> From: Prussin, Calman (NIH/NIAID) [mailto:CPRUSSIN@niaid.nih.gov] 
> Sent: Wednesday, February 16, 2005 11:40 AM
> To: Cytometry Mailing List
> Subject: Surface markers for gut epithelial cells
> 
> We are studying T cells in G.I. biopsies by flow and want to clean up the
> noise due to non-hematopoietic cells. I suspect that most are epithelial
> cells. Does anyone know of a good cell surface marker to identify gut
> epithelial cells? I am hoping to use it in my dump channel. Thanks.
> 
> Calman
> > _______________________
> > Calman Prussin
> > Laboratory of Allergic Diseases
> > NIAID/ National Institutes of Health
> 
>