>Quick question: We are using a FACSAria with a 407nm laser. We are >wanting to get a dye >that is excited at 407nm and is as bright as possible (when >conjugated to an antibody >with MP's Zenon reagents). The current PMT filters are at 530/30 nm >and 450/40 nm. We >could change those if that would help. > >We have tried Alexa 430 and found it to be rather dull. Any >thoughts about whether Alexa >405 or Cascade Blue would be any brighter? Alice- We have had good results with Alexa 405 and cascade blue. Commercial conjugates of these appear to vary in quality, though. The best staining we've seen is with Pacific Blue. These have been run on a DiVa with 200mW 407 nm and on an LSR with 25 mW 405 nm. I would expect poorer results (but usable) on the Aria since the supplied violet diode is lower power and there is significant loss in the fiber delivering the laser to the flow cell. All of the above measurements used a 450/40 bandpass. Marty Marty Bigos Director, Flow Cytometry Core Laboratory Gladstone Institute of Virology and Immunology Mail: 1650 Owens Street San Francisco CA 94158 (tel) 415-734-4821 (fax) 415-355-0855 mbigos@gladstone.ucsf.edu http://www.gladstone.ucsf.edu http://www.gladstone.ucsf.edu/gladstone/php/?sitename=flowcytometry > >Thanks. > >Alice > >Alice L. Givan >Englert Cell Analysis Laboratory >of the Norris Cotton Cancer Center >Dartmouth Medical School >Lebanon, NH 03756 USA >tel 603-650-7661 >fax 603-650-6130 >givan@dartmouth.edu >www.dartmouth.edu/~celllab --Received on Wed Feb 2 19:47:30 2005
This archive was generated by hypermail 2.1.8 : Sat Jan 14 2006 - 22:03:35 EST
