Dear Alice, Another alternative to Alexa405 or Cascade Blue (which I found not to be very quantum efficient either) is Molecular Probes Pacific Blue. Regards Gary Warnes At 3:12 PM -0500 1/28/05, Alice L. Givan wrote: >Quick question: We are using a FACSAria with a 407nm laser. We are >wanting to get a dye >that is excited at 407nm and is as bright as possible (when >conjugated to an antibody >with MP's Zenon reagents). The current PMT filters are at 530/30 nm >and 450/40 nm. We >could change those if that would help. > >We have tried Alexa 430 and found it to be rather dull. Any >thoughts about whether Alexa >405 or Cascade Blue would be any brighter? > >Thanks. > >Alice > >Alice L. Givan >Englert Cell Analysis Laboratory >of the Norris Cotton Cancer Center >Dartmouth Medical School >Lebanon, NH 03756 USA >tel 603-650-7661 >fax 603-650-6130 >givan@dartmouth.edu >www.dartmouth.edu/~celllab -- Dr. Gary Warnes FACS Laboratory Cancer Research UK 44 Lincoln's Inn Fields London WC2A 3PX P: 020 7269 3394 F: 020 7269 3100 mail to:gary.warnes@cancer.org.uk Charity No: 1089464 OCTOBER IS BREAST CANCER AWARENESS MONTH To find out more visit www.cancerresearchuk.orgReceived on Tue Feb 1 12:58:01 2005
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