Sorting 3T3 cells

We have been experiencing a situation where we see  a lot of cell death
after sorting 3T3 cells.  The cells prior to sorting seem to be in good
shape, good viability etc. but following sorting we see a change in the
scatter parameters and most of the sorted cells are dead within 24 hrs.

Our sorting conditions have varied, but the most current set are as
follows.  We are sorting on a FACStarPLUS with a 100um nozzle, primary
laser 488nm at 200mw sheath pressure at 8 PSI.

Has anyone else noticed that 3T3 may be more susceptible to the rigors of
sorting??

Charles A. Kuszynski, Ph.D.
Assistant Professor/Director
Cell Analysis Facility
University of Nebraska Medical Center
985816 Nebraska Medical Center
Omaha, NE 68198-5816
402 559-6299 office
402 559-6267 lab
402 559-4077 fax
ckuszyns@unmc.edu

***The University of Nebraska Medical Center E-mail Confidentiality
Disclaimer***
The information in this email is privileged and confidential, intended only
for the use of the addresse(s) above.  Any unauthorized use or disclosure
of this information is prohibited.  If you have received this email by
mistake, please delete it and immediately contact the sender.