Purdue Cytometry Mailing List By Subject
- Howard Shapiro (Wed Sep 29 2004 - 22:40:33 EST)
- "A stranger is a Friend I have not meet yet"./Spectral Overlap
- "Analysis of Microbial Cells at the Single Cell Level"
- "Analysis of Microbial Cells at the Single Cell Level" - Correct Address
- "Cellular Astronomy" - The Next Small Thing?
- % of Lymphocyte by hematology counter vs. FLOW
- % of Lymphocyte by hematology counter vs. FLOW: FOLLOW UP
- (almost) sterile sorting
- (anti) Commercial Posting:)
- (Fwd) CFP and Announcement - International Journal of Medical
- (Fwd) Positions to post
- (no subject)
- 10th Leipziger Workshop, 2nd announcement
- 1301 telomere length
- 14-bit ADC to 18-bit scale... why bother?
- 17th Annual Clinical Flow Cytometry Course in Pittsburgh, Sept 5- 10
- 2004 - A quality year in Cytometry: Review
- 200u sorting on VantageSE
- 200u sorting summary
- 3D Graphics for flow data display
- 405 EXCITABLE DYES
- 405 nm laser // time lapse microscopy
- 405 nm vs. 532 nm laser
- 488/UV multiline laser
- 4th EWGCCA EuroConf, Sep.23-25, Mol, Belgium
- 5 color flow
- 5 or 6 colour reagents/panels on CYAN
- 532nm Excitation
- 6-NBDG, advice needed
- 600th person does survey
- 7AAD
- 7AAD : other viability options
- 7AAD and pyronin Y
- 7AAD or PI with APC, PC7, APC-Cy7 conjugates
- 96-well plate autosampler
- 96well PCR plates on the Aria
- =??Q?=BB=D8=B8=B4: Activation of platelets?=
- ?BLASTIC NK LYMPHOMA/LEUKAEMIA
- [ CSF1 and PDGF receptor levels? ]
- [ Fast sorter recommendation? ]
- [bacterial stably expressing GFP/YFP?]
- [BRdU in LOVO cells]
- [CD57 on T Cells]
- [Cell Cycle phase in subpopulations]
- [Compensation with different sized populations]
- [Cytometer Purchase advice?]
- [DNA dye exclusion viability test question]
- [DNA Staining of cells with intracellular bacteria]
- [Functional Analysis ISAC Workshop]
- [Fwd: N2 assay]
- [Hoechst / CD34 protocol ? ]
- [ISAC Workshop - apoptotic signal transduction]
- [MCSF-1 and PDGF receptors on smooth muscle cells]
- [Memory B cell in CVID]
- [MFI vs. percent positive ? ]
- [Neutrophil functional markers?]
- [Platelet analysis in from whole blood samples]
- [Reagent source?]
- [Sapphire laser opinions]
- [Surface marker quantitation questions]
- [TRUCOUNT for mouse WBC?]
- ```NK cells viability post sorting on FACSAria
- A Little Help From my Friends/ Spectrlal overlap
- A manufacturer's view of the instrumental calibration controversy
- a new home for an old Elite
- A Tutorial on WinMDI
- Ab PHAGE LIBRARY
- about the marker for RAG -/- mice
- about the time point in the BrdU cell cycle experiment
- absolute vs ratio, small comment
- Abstract Deadline for the Clinical Cytometry Meeting AUG 15th
- Academic Institution User Fee Survey Revisited
- ACAS Ultima confocal laser cytometer/SerCon
- ACAS Ultima confocal laser cytometer/SerCon-service rep
- Accutase for detachment for cell monolayers
- ACDU Error Rate
- Acridine Orange
- Activated neutrophils/eosinophils?
- Activation of platelets
- Adult peripheral blood
- Advanced Courses in Flow Cytometry in NY City in September
- Advice needed
- Advice on how to prepare adipocytes from rats to perform flow cytometric analyses
- AGGRESSIVE ?LYMPHOMA CASE
- Alexa Fluor 680/700 HeNe excitation
- Alice Givan and Howard Shapiro Seminar at UMass
- alignment of Coulter Elite
- Alkaline Phosphatase staining
- Aloha...and thanks!
- Alpha Beta TCR staining
- alternate source for Ortho Permeafix?
- alternative T cell activation markers
- ALTERNATIVE T-CELL ACTIVATION MARKERS
- an update
- Analisis of cell proliferation with CFSE
- analysing ROS?
- analysis of mixed primary cultures of neurons
- Analysis Software
- analysis vs sorting and nozzle sizes: FACSAria
- analyzing nuclear proteins by flow cytometry
- Andy Beavis - are you out there?
- Annexin binding on platelets
- Annexin on PBMC - real or false positives?
- Annexin on PBMC - real or false positives? - Summary of responses
- Annexin staining with platelets
- Annexin V
- Annexin V and caspase 3 protocol
- annexin V and monos
- annexin V on monocytes
- annexin V staining of peripherAL BLOOD MONONUCLEAR CELLS
- Annexin V vs. VAD-FMK
- Announce: Multiplex Assay Meeting UK
- Announcement, 10th Leipziger Workshop
- Annual Research Course in Flow Cytometry
- Another Acridine Orange Question
- Another CDC ORISE position
- anti Pichia antibodies
- anti-Flk-1 antibody options
- anti-human Notch1 antibody for flow
- anti-human-alpha4beta7
- anti-IL-6 antibody against not-recombinant IL-6
- anti-mouse CD18 and CD45
- anti-mouse FasL antibody
- anti-rat CD19 cross reacting mAb
- Anti-sheep MoAbs
- anti-TGF-bata antibody
- anti-TNF antibody
- antibacterial peptides
- Antibody against EPO
- antibody conjugated APC-Cy7
- Antibody dilutions
- antibody for flow-FISH
- Antibody Request
- Antibody titrations (2004)
- Antibody titrations (2004...2005...2006... and beyond)
- Antibody titrations-1 question
- Antibody-based uptake assay
- Antwort: Gating for apoptosis
- Antwort: Green fluorescent stain for K562 cells
- Antwort: maximizing recovery of paraformaldehyde-fixed cells
- Any experience of TRUE undifferentiated acute leukemia?
- Any one working with mouse blood?
- Any one working with mouse blood? -correction
- Any suggestion for selection of ICAM-1 (CD54) antibody?
- anybody using the Chroma Technology filters?
- Anyone used DiD
- Anyone using Cellquest on a Mac.
- anything better than Alexa 430?
- APC
- APC-Cy7 conjugated Streptavidin
- APODIRECT flow cytometry kit (Phoenix Flow Systems)
- apoptosis
- Apoptosis analysis
- Apoptosis and trypsin
- apoptosis in fresh PBMCs
- Apoptosis in peripheral blood lymphocytes (Tunel assay)
- Apoptosis Workshop-ISAC
- Apoptosis, Tunel assay in CD4 and CD8 T cells
- Applescript and CellQuest Pro
- Aramark CAPP
- Aria ideal max. sort rate w/ viable cells
- Aria medium and low pressure sorting
- Aria nozzle issues revisited
- Aria nozzle summary - Thanks
- Aria nozzles
- Aria sorting into 96 well plates
- Aria Stability
- Aria Users please read:
- Aria-ACDU adaptor for PCR rack?
- aseptic sort set up on Aria
- ask the Profis
- aspecific PE-staining on neutrophils
- AT1 expressing cell line.
- ATG and OKT3 determination / depleting
- Autoflourescence as "control" - finally an answer?
- Autofluorescence blocking
- Autofluorescence quenching
- Autofluorescence quenching - Clarification
- Autosampler for Facscalibur with temperature control - alternatives to Cytek?
- AW: Beads for cell size measurements
- Azide and calcium flux
- B-cell Markers on EBV Transformed B-Cell lines
- Bacillus spore permeabilisation and viability assessment
- Back doors to Overlays
- bacteria & low pH / high salt
- bacterial permeabilisation
- Basic and Advanced Flow Cytometry Courses in Denver
- Basic problem with RBC lysis
- Basic rhesus macaque cytometry
- Bayer Flow Cytometer
- BD CompBeads
- BD FACS Caliber
- Beads
- beads / cells/ml
- Beads for cell size measurements
- Beckman Coulter XL
- Best Secondary Reagents for Rabbit Polyclonal Abs
- Bio-containment
- BioE Vitalyse
- Biohazard containment
- biohazard sorting on Aria
- biohazard sorting on Aria - follow up
- Biohazardous sorting on an Aria
- BioRad Bryte HS
- Biorad confocal users
- BiOS 2005 Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II (BO124).
- BiOS Meeting Announcement
- Biosafety and sorting infectious samples
- Biosafety of sorting revisited
- blood CD154 staining
- Blue laser Guava
- BM Biopsy for Flow
- bone marrow core biopsy processing
- Boston Users Group for Cytometry / CFAR HIV Meeting Announcement
- Boston Users Group for Cytometry HIV Meeting Announcement
- Boston Users Group for Cytometry Web Site
- Boston-Flow Cytometry Job Opening
- Bragging rights....!!
- brains
- Bravo, Bravo ISAC XXII
- BrdU
- BrdU and activated T cells in vivo
- BrdU and H33342
- brdu and saponin
- Brdu PI
- BrdU staining and surface antigen
- Breast milk lymphocytes
- Bug/feature in FC500 File naming - loss of data
- c-myc anti-mouse mAB
- Ca flux in frozen PBMC
- Ca for loading cells
- Ca-Flux
- caco cells and bacterial attachment
- Calcium ratio imaging events
- calibration beads analisis
- Calibration emission lamp for spectral confocal QA
- Calibur Crash Summary
- Calibur Crashes
- Can cells for flow be frozen?
- Can I do intracellular staining with biotin antibody ?
- Can some one offer an answer to my problem
- CAP checklist question FLO.30720
- Cardiomyocyte marker?
- Cardiomyocytes
- Carry over of fluorescent probes and stains to subsequent samples
- Cascade Cytometry User's Group meeting on Jan. 4
- Caspase activity in lymphocytes
- caspase activity in lymphs
- CBA quesion
- CBA question
- CBA versus flowcytomix
- CBA versus flowmix
- CCR3 and CCR10
- CCR5 mAb that works on fixed cells
- CCS meeting coming up
- CD 8
- CD antigen question
- CD133 Antibodies
- CD133 in paraffin/frozen tissue sections
- CD15 staining...
- CD16 in cynomolgus monkeys
- CD1a+ cells in LBA
- CD27 Staining
- CD3-CD4+
- CD34
- CD4 and CD8 coexpression problem
- CD4+ T cell enumeration using non-traditional cytometers
- CD4+//CD8+ in apparently normal individual
- CD4+CD25bright
- CD4-CD8- T cells in pediatric AIDS
- CD4/CD45RB sorts - High speed sorting
- CD45 expression in mouse lung
- CD45 versus SSC in mice bone marrow: summary
- CD64
- CD69 timing
- CD8 availability?
- CD8 available upon request
- CD94 and dog NK cells
- CDw32+ L cells
- CEDA,SE to replace CFDA for cell division
- cell activation markers
- cell alkaline dissociation for the chromosome aberration analysis
- cell clumping
- Cell Counter
- cell cycle analysis
- Cell Cycle Analysis by Centrifugal Elutriation
- cell cycle analysis in 96 well format
- cell cycle analysis of Jurkat
- Cell cycle analysis with DRAQ5
- Cell Cycle on Aria
- cell cycle on Aria answers
- Cell fixation after Annexin V labelling ?
- cell line on FACScalibur
- Cell size and FSC
- cell sorter and stem cell
- Cell sorter for sale (UK)
- Cell sorting with viability !! will it be possible?
- Cell Tracker Orange Alternative
- Cellquest Pro files into Cellquest
- CellQuest Pro Question
- CellQuest problem
- CellTrace Far Red for in vivo cell tracking
- Cellular Astronomy
- CFDA SE compensation control
- CFP and YFP beads
- CFP on LSR II/Qdots
- CFSE
- CFSE part 2
- cfse quenching?
- CFSE staining for spleen/lymph node cells
- cheapish books
- cheapish books AND Not so cheapish ones.....
- Check out the latest ISAC blog!!!
- Chicago User's Group (ChUG)
- Chris Threeton
- Clinical Flow Course Pittsburgh Sept 5-10
- Clinical Flow Cytometry Course
- Clinical Flow Cytometry Instrument - *Please Respond*
- Clinical Flow Cytometry Instrument - Please Respond!
- Clinical Flow Cytometry Instruments
- Clinical Flow Cytometry Position
- Clinical Flow Techologist Position
- CML and sorting
- CML panel
- COLD AGGLUTININS
- Column dye
- Comments I have recieved about ZAP-70
- Commercial polyclonal antibody prep
- Compbeads and Zenon kits
- Compensation Question
- Compensation Question Summary
- compensation videos
- COMPETITION COMMISSION CLEARS ZEISS/BIO-RAD MERGER
- Complimentary subscription of CELL CYCLE to some members of the scientific community
- CompuCyte
- Computer needed
- conferences
- Confocal Instability-shocking observation
- Confocal microscope Instability
- Confocal microscope Instability #2
- Confocal QA system stability --Summary responses
- Confocal Shape changes in Round beads
- Confocal spectral calibration standards
- Conjugation of Anti-Rat Antibodies
- Consultant needed
- Contract lab needed
- control diploid used in cell cycle analysis
- cord blood staining
- Core Manager Position-Chicago
- Core Manager's Meeting announcement
- Core Manager/director Survey
- Core Managers benchmark survey request
- Core recharge strategies for new instrumentation
- Correlation between cell viability/cytotoxicity studies
- Costar 50 and 100ml reagent reservoir
- costar reservoirs
- Coulter counter for sale
- Coulter FC500 versus BD FACSCalibur
- Coulter XL - FlowJo question
- Cox-1 & Cox-2
- CPT codes
- CPT coding for CD4/CD8, revisited.
- Creating a database of FCS files
- cryopreservation of lymphocytes for flow analysis
- cumulative apoptosis
- Current protocols in Cytometry
- custom-conjugation of antibodies
- Cy-Chrome-detection
- cy5 antibody
- CyFlow counter from Partec
- Cytometry CDrom Volume 8 - almost last call....
- CYTOMETRY IVth Edition
- DAF-FM diacetate and NO in EC?
- Daily optimization
- Data storage on DVDs
- Daudi Cell Line in mice
- DC linage 2 stage protocol?
- Deadline approaching for FloCyte Advanced Flow Cytometry Course in Madison, WI
- Deadline April 16 for Flow Course in NYC
- Deadline for Adv. Flow Course in Madison
- Deadline for FloCyte Regional Training Program Courses in San Diego - July 21
- Dear all,
- Debris in sample prep
- deciding if 2 pops are the same, and if negative.
- Definition of Near-diploid in breast cases
- Dendritic cell lines...
- Dendritic cells
- Detecting Intracellular IL-10/IL4
- Detecting microchimerism in blood
- Detection of effector from effector memory cells in rat
- Detection of Sepahrose beads by FCM
- detection of smad proteins
- Detection of Viruses on a FACSCalibur?
- Detroit Users Group Meeting 6-18-04
- Dichroic filters
- Dick Hoagland's current email address?
- Difference in Rho-123 fluoresence
- differential cell count - mouse peritoneal lavage cells flow staining
- Digital Channel Number
- digital optical web site
- dilution effect?
- DiOC / PI question
- Disaggregating Needle Biopsy
- Discovery Image Analyser from BD
- Discrimination of GO/G1
- Discussion list summaries
- Discussion of a Human Cytome Project at the E.M.C.
- Display problems with CQ Pro OS X
- Displaying Indo-1 data
- Distance Learning Course
- DIVA
- DIVA 4 auto compensation issue?
- DiVa 4 Summary
- DiVa 4.0 FCS export
- Diva 4.0 files
- DiVa 4.0 Software
- DiVa 4.0 users question
- Diva for sale
- DiVa ND filter holder modification?
- DiVa problems
- DIVA problems? Calcium anyone?
- DiVa v4 problems
- DiVa-FlowJo axis events
- DiVa/Turbo air source?
- DiVa/Turbo help anyone?
- DNA Analysis on Archival Breast Tissue
- DNA cell cycle data Question
- DNA content and DNA index for zoo animals
- DNA content of nuclei from dried plant tissue
- DNA extraction from fixed cells
- DNA extraction from formaldehyde fixed cells
- Dna fragmentation in sperm cells
- DNA ploidy analysis of seed
- Do your have any DIVA problems?
- Does anyone have Dr Carl Stewart's email address? His last one (thank god for the train) bounced.
- Double cytokine staining of cells
- Down regulation of human CD8+
- dr. mr. on Apple
- DRAQ5
- DRAQ5 Source in US?
- DsRed
- DsRed beads?
- East Africa Cytometrysts
- ECFP analysis on Vantage with 457nm line (secondary laserline)
- Ecological question: how to differentiate between species by their pigmentcomposition?
- editing list mode data file
- EDTA solution
- Educational Opportunity
- Effect of time on viability
- Effects of freezing and DMSO on expression
- EGFP Calibration beads and BD LSR-I Machine?
- ELISA on polystyrene microspheres
- ELISA vs CBA
- Employment Opportunity
- Endothelial cells
- Endothelial cells summary
- endothelium
- Eosinophil analysis
- EPICS V Sample Caps
- essential Aria part
- Estimados colegas y amigos de Madrid y Espańa
- ethidium monoazide and annexinV
- evaluating in house conjugated dynal beads
- Evan's Blue Dye
- Excess Flow Cytometer
- Excluding dead cells on phagocytosis assay.
- Experience with G0/G1 in live cells with flow
- Experiences with Cytek Time zero system for Calcium flux
- Expired antibodies
- EXPIRED ANTIBODIES FOR FLOW
- exporting raw data from gated events
- External Proficiency Testing
- Extinction Coefficient of Quamtun dots
- eYFP and 7AAD
- FACASARRAY
- FACS Analyzer to go
- FACS Aria and LSRII Red Laser
- FACS Aria compatible software
- FACS Aria Compensation
- FACS data analysis