Antwort: Green fluorescent stain for K562 cells

Hi Paul,
we have successfully used green fluorescent PKH2 (PKH2-GL kit from Sigma,
final labeling conc. 1 µM and 1e7 cells/ml, 5 min. incubation at RT) to
label A431 cells for a cluster assay which was used to study antibody
mediated aggregation of EGFR+ A431 tumor cells and (PKH-26 labeled) FcR+
U937 cells (mimicking peripheral blood effector cells).
Best regards
Georg

Hans-Georg Kreysch, Dipl.-Ing.
Global Technologies / CADS
Merck KGaA, Darmstadt
Phone: +49 6151 72 7323
e-Mail: hans-georg.kreysch@merck.de




|---------+---------------------------->
|	  |	      Paul Connellan   |
|	  |	      <pconnell@scu.edu|
|	  |	      .au>	       |
|	  |			       |
|	  |	      23.12.2004 01:19 |
|	  |			       |
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  |	  An:	    Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>		 
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  |	  Thema:    Green fluorescent stain for K562 cells				 
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Hi,

For the NK cytotoxicity assay, I would like to label the K562 target cells
with a green fluorescent label
Ideally the stain should have minimal impact on cell viability & negligible

transfer to other cells as I incubate PBMCs with the K562 target cells then

determine percentage dead target cells using propidium iodide.

Could anyone suggest an appropriate dye ?

Thanks
Paul
---------------------
Paul Connellan
Research Associate
Natural Products Pharmacology Unit
(Joint Venture ACCMER/CPP)
Centre for Phytochemistry & Pharmacology,
T Block,
Southern Cross University,
Lismore NSW 2480

Phone: +61 02 66223211
Fax:	      +61 02 66223459

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