Hello fellow flowers- I am looking to detect F-cells via flow. I have read many papers indicating that you can do this by IC staining with anti-HbF. I have only read and performed IC staining following some sort of fixation (i.e. gluteraldehyde or formaldehyde) and permeabilization. I have 2 questions: 1. Is is necessary (and if so, why) to fix cells before permeabilization? ( I believe the answer to to perserve cell morphology and prevent "leakage" of IC proteins, but I wonder if there is another more important reason). 2. Has anyone heard of another method of detecting F-cells beyond anti-HbF IC staining? Any cell surface marker known specifically for F-cells? I am interested in performing cell sorting and isolating proteins,thus the fixation process will be a huge issue in down stream applications. With much appreciation- Karis Hughes karismhughes@yahoo.com Karis M. Hughes University Texas-Dallas Graduate Student karismhughes@yahoo.comReceived on Mon Dec 20 12:58:00 2004
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