Hello, We are planning on looking at viability of cultured cell lines using the YO-PRO-1 stain from Molecular Probes in conjunction with propidium iodide. This analysis requires that the cells remain unfixed. Our flow cytometer is not on-site, it is located approximately fifteen minutes away. What is the general opinion on how long cells can be detached from culture-ware prior to analysis without significantly affecting viability measurements? Any suggestions on how to minimize damaging effects on cells during this time would be appreciated as well. Thank-you for your help, Brian Brian Sullivan Vincent Center for Reproductive Biology Massachusetts General Hospital btsullivan@partners.org --------------------------------- Do you Yahoo!? The all-new My Yahoo! – Get yours free!Received on Thu Dec 16 13:38:01 2004
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