RE: Fixing Cells, after hour use

----Original Message-----
From: Pamela Shaw [mailto:pshaw@spahs.umt.edu]
Sent: Tuesday, December 07, 2004 6:22 PM
To: cyto-inbox
Subject: Fixing Cells, after hour use

  I suggested he fix the cells so we could run them at a reasonable hour and
also for bio-safety reasons.  Without getting into lengthy bio-safety issues
again, can anyone tell me if there is a reason why we should not fix these
cells?	He claims on the last experiment where I had him fix the cells, he
did not get good results. (Cells were fixed with 2% paraformaldahyde. The
product insert indicates fixing is acceptable.)  


     My suggestion would be to run the cells live next time, and if there is
sample left over, fix it and run it the next day.   Then you will have the
experiemtnal evidnce for fixation, provided it actually is ok for these
cells and this assay system.


regards,

Tom


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* 
Thomas W. Mc Closkey, Ph. D. 
Scientist in Charge, Flow Cytometry Facility, North Shore-LIJ Research
Institute 
Assistant Professor, Pediatrics, New York University School of Medicine 
Biomedical Research Center, 350 Community Drive, Manhasset, Long Island, New
York 11030 
ph:  516-562-4844 [office], 516-562-1135/4641 [lab]  fax:  516-562-2866 
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