Fresh apoptotic PBMC are easily detectable by TUNEL assay(e.g. Gorczyca et al.,Induction of DNA strand breaks associated with apoptosis during treatment of leukemias. Leukemia, (Baltimore) 7: 659-670, 1993). Likewise, the activation-induced apoptosis of lymphocytes is best detected using the TUNEL assay (e.g. Halicka et al., Enhancement of activation-induced apoptosis of lymphocytes by the cytotoxic ribonuclease onconase (Ranpirnase). Int. J. Oncol., 21:1245-1250, 2002). However since no apoptotic cells are present among PBMC just after their isolation from normal healthy individuals, therefore no TUNEL positive cells can be detected unless one induces apoptosis e.g. by culturing for 3-5 days Zbigniew Darzynkiewicz -----Original Message----- From: Marjan [mailto:gharagozlo@pearl.sums.ac.ir] Sent: Friday, December 10, 2004 1:08 PM To: cyto-inbox Subject: apoptosis in fresh PBMCs Dear All Have you ever examined apoptosis in fresh PBMCs? I read in some articles that apoptosis in fresh PBMCs can not be detected by Tunel test; the cells should be cultured for 3-5 days before apoptosis test. What is your idea? I would be grateful if you could kindly give me the benefits of your experiences. Looking forward your kind reply Best Marjan Sincerely yours: Marjan Gharagozloo (Ph.D student) Shiraz Institute for Cancer Research Shiraz, Iran PO Box:71345-3119 Phone:+98-913-1100447 Fax:+98-311-6259769Received on Mon Dec 13 14:58:00 2004
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