Greetings, I have an investigator who has asked me to stay very late (midnight) to run timed assays on more than one occasion. He is looking for Caspase activity in human macrophages from lung lavage. I suggested he fix the cells so we could run them at a reasonable hour and also for bio-safety reasons. Without getting into lengthy bio-safety issues again, can anyone tell me if there is a reason why we should not fix these cells? He claims on the last experiment where I had him fix the cells, he did not get good results. (Cells were fixed with 2% paraformaldahyde. The product insert indicates fixing is acceptable.) He was not specific, so I don't know what he meant by it, or if it had anything to do with fixing. Core managers, what are your policies on running samples after hours? We do allow independent operators to run after hours, but these particular investigators are not trained at this point. I'm a bit reluctant to train them as they don't log enough hours for me to feel comfortable doing that yet. Pam Shaw Fluorescence Cytometry Core Facilitator Center for Environmental Health Sciences The University of Montana 32 Campus Dr, Skaggs Building 052 Missoula, MT 59812 Ph (406) 243-4545 email: <mailto:pamela.shaw@umontana.edu> pamela.shaw@umontana.edu www.umt.edu/cehs <http://www.umt.edu/cehs>Received on Wed Dec 8 16:18:00 2004
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