A combination re the above configuration that would detect APC-Cy7, APC-Cy5.5 and APC is as outlined below APC-Cy7 (emission 780) Trigon position A Dichroic 755LP Band pass 780/60 APC-Cy5.5 (emission 695) Trigon position B Dichroic 675LP Band pass 690/15 APC (emission 660) Trigon position C Dichroic Blank Band pass 660/20 The reduction of the APC-Cy7 signal needs to be checked out , by what criteria are you saying the signal is reduced , also to what magnitude ? A nice way to evaluate the signal detection for each of the PMT's is to use some Comp Beads with the relevant fluorochrome on each one then run them and verify the detection of signal in each PMT, it is a good simple way to start . IanReceived on Tue Dec 7 13:38:00 2004
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