Purdue Cytometry Mailing List
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Starting: Wed Jan 01 2003 - 01:38:00 EST
Ending: Wed Dec 31 2003 - 14:18:01 EST
- "Break in period" question
- "cell tracker" for light microscopy
- "permanent" label
- "What is life?" ... you should always know!
- 'Correction' or 'Corrigendum' (?)
- (no subject)
- 1st International Cytomics Meeting, Newoport Wales, Today!!!
- 2',7'-dichlorofluorescin diacetate
- 2',7'-dichlorofluorescin diacetate photodecomposition
- 2',7'-dichlorofluorescin diacetate; Response
- 2-color compensation questions
- 20% MSA Discount on PFC4 Good Through Oct. 31
- 2002 was a good year in Cytometry- 2003 will be a great year!
- 2003 A Review from the Purdue Cytometry Discussion
- 2003 Cytometry Development Workshop
- 2003 Flow Course Announcement
- 27th ANNUAL COURSE IN FLOW CYTOMETRY - June 12-18, 2004 - Bowdoin College - Brunswick, Maine USA
- 3D Graphics for flow data display
- 4 colour stain problems
- 45 as alternate
- 4c combinations
- 5 and 6 color analyzers
- 5 color flow
- 5% ETOH as apoptisis inducer?
- 5% ETOH Summary
- 5+ Color capability
- 7 to 9 color facs
- 7-AAD
- 7AAD Staining
- 8. Leipzig Workshop, Practical Course Application
- 8. Leipziger Workshop, Deadline
- 88342 and 88180
- 8th Leipzig Workshop, deadline
- 96 well filter plates in MAS
- 9th Leipziger Workshop, 2nd Int. Workshop Slide Based Cytometry
- :Lasers for parts or rebuild.
- [7-AAD non-specific binding?]
- [Aerosol containment for Vantage?]
- [AlexaFluor tandems?]
- [anti-mouse prolactin receptor Ab?]
- [Anyone need Tektronix Phaser consumables?]
- [AO for retics experience?]
- [BrDU + PI after irradiation]
- [Discriminate fused cells and fused nuclei]
- [e-mail address request]
- [Ficoll to remove debris?]
- [FITC / Crystal Violet phagocytosis assay]
- [flow] reading DIVA FCS files with cellquest
- [Fwd: calcium studies on mitochondria]
- [Fwd: Drosophila sorting and viability]
- [Identifying participants]
- [Jet in air biosafety]
- [Losing HPAECs during prep]
- [Mac OS X software?]
- [Macrophage phagocytosis]
- [mito-tracker protocol question]
- [MulticycleAV]
- [Optilyse C problem]
- [Phagocytosis control problem]
- [Plant cell suspension prep]
- [Plant cell suspension technique]
- [Quantum Dots]
- [SH-SY5Y differentiation]
- [Sorting pollen grains]
- [Three-color question]
- [WTB MoFlo]
- A big Thank You
- A Cell Fixation Efficiency Question
- a different co-authorship question
- A friend of Dick's
- A little help from my friends Cy-3/LSR
- A mixture of two vital stains.
- A sticky problem with macrophages
- AAI Meeting 2003
- ab capture beads
- Ab's to rat IL-4 & IL-6
- Ab's to rat IL-4 & IL-64
- Absence of CD33 expression on all myeloid cells
- absence of myeloid markers...
- ACAS 570 for sale
- Advanced Training in Flow Cytometry in the Southland
- advice on CFSE staining
- advice on sorting a population of vesicles
- Advise for apoptosis
- AFCG 2003 Meeting
- AFCG 2003 Scientific Meeting and Workshops
- AFCG Meeting Notice
- alexa 568
- alexa 660 and percp 5.5
- Alexa flour 488
- AlexaFluor conjugations
- AlexaFluor488/Sorting
- Alignment beads
- Alignment beads [SB-5IIV8P2]
- Alizarin Red?
- all on scale?
- All the best
- alpha4beta7
- alternate source for Ortho Permeafix?
- AML M7 PROFILE
- AMLI Clinical Immunology Meeting
- An ASP at my breast
- An ASP at my breast: Outcome
- An isopycnic method for keeping cells in suspension
- Analysis of Pituitary Cells
- Analyzing fluorescence and/or properities per cell
- analyzing live Microcystis aeruginosa
- analyzing sub G1 populations for apoptosis
- analyzing toxin producing cyanobacteria [DC-EAEJ1V3]
- Andrew Riddell
- Angles of detections
- Annexin control
- Annexin control continued
- annexin v
- Annexin V APC
- annexin V controls
- Annexin V/ PI kit
- Announcement of a Quantitation Tools Workshop
- another cell fixation question
- Another great discounted Book -
- another nylon mesh source
- another salary survey for comparison
- Another Survery - Future of Cytometry......
- anti-carbonic anhydrase
- anti-CD20 treatment
- Anti-D Immunoglobulin prophylaxis and anti-D reagent
- anti-hamster antibodies
- anti-HLA & anti-platelet antibodies
- anti-HLA-A3 for flow
- anti-m IFNa
- Anti-mouse CD87 (uPAR) Ab
- Anti-mouse IL-13?
- anti-mouse Langerin
- antibodies
- Antibodies cocktail for basophil identification in mice
- Antibody combination validation
- antibody specific for beta-galactosidase
- antibody stability
- antibody titration
- Antibody's dosage problems (interseting)
- Antibody-loaded magnetofluorescent liposomes
- antiBrdU-FITC/antiCD4-PE/antiCD8-PerCP-Cy5.5 combination
- Antigen Retrieval
- Antwort: [Losing HPAECs during prep]
- Antwort: Annexin control continued
- Antwort: trypsinization and cell surface proteins
- Anyone familiar with "pevikon block preparative electrophoresis"
- apoptosis and Bcl-2
- Apoptosis and Ficoll-Hypaque
- apoptosis and intracellular cytokine stain
- apoptosis and loss of membrane potential
- Apoptosis Detection by PI-dim
- Apoptosis inducing agents
- Apoptosis-cytometry with MDCK
- Apoptotic bodies
- Are Quantum Dots toxic?
- area under curve
- Argon UV vs Krypton UV
- Argon UV vs Krypton UV [HS-EU6JF63]
- ASTHMA AND ALLERGIES IN POSTWAR KUWAIT
- autofluorescence in macrophages
- autoreplies
- AW: Extracting data from FACS measurements
- AW: FACS vs MACS
- AW: Help us on PMT settings on the Aria
- AW: keeping cells in suspension
- AW: Small particles
- AW: Sort Watch
- AW: URGENT HELP NEEDED / nano-particles
- Azide
- Azide/Preservative-Free Ab conjugates
- B7-DC
- background expression with pEGFP-1 vector
- Bacterial viability
- Basic and advanced Flow training in 3 locations
- basic chemistry question:serum+EDTA?
- Basic Flow Cytometry Course in Southern California or Tampa Florida area
- basic physics of high speed sorting
- Basophil Degranulation and Infinite Dilutions
- Basophil Degranulation and Infinite Dilutions [NVC-D1W9992]
- BASOPHIL DEGRANULATION/ASTHMA
- Bay Area Compensation Workshop
- Bblood Typing
- BD ARIA Nozzle
- BD cytokine bead array data file transfer
- BD FACSCanto
- BD vs. Coulter
- Beads for Fluorescence normalization, What kinds?
- Beads in Soil Samples
- beads, yet again
- Beckman Array System Disks
- BENCH TOP FLOW CYTOMETRY COURSE
- best protocol for cell cycle analysis
- Big cell sort
- binding of fluorocrome to Ig
- Biocontainment in the Aria
- BioRad Bryte HS
- Biosafety in the laboratory: working with infectious material (was: Sorting live human lymphocytes) [DC-RRGX173]
- Biotech Job Openigs in Melbourne, Australia
- BL3 sorter cost
- Blank emails from the Cytometry Mailing List
- blocking anti-IgE
- Blocking of Fc receptors
- blood grouping
- Blood Typing
- bone marrow
- Bone marrow cell distribution
- Books
- Boston Users Group for Cytometry Multicolor Compensation Meeting Announcement
- bovine TLR
- Brain nuclei isolation for flow cytometry
- BrdU
- Brdu labelling
- BrdU staining
- BRDU/Dnase
- BRDU/PI
- Breast Cancer and flow
- c-myc anti-mouse mAb for FACS
- C:biovolume ratio
- Ca++ flux problem
- Ca++ flux problem - Summary 2
- Ca++ flux problem - summary of responses
- Ca2+
- Calcium calibration
- Calcium Flux Positive Control for Mast Cell Line
- Calcium on a Vantage
- Calcium on a Vantage [BP-2373LG4]
- Calcium on LSR
- calcium studies on mitochondria
- calculation of F/P ratio
- Calibration beads & fixatives for DSRED
- Calibur Questions
- Calibur Wanted
- Cambridge, MA flow cytometry position
- Can light be "stolen"?
- Canadian FACS facilities only
- Canuck Flowers at CCS
- CAP Inspection Questions
- CAP Requirements
- CAP Survey
- CASP-Glow Red
- CCD camera linearity
- CCD camera linearity for image analysis (ELISPOT)
- CCR5 clumping in LWB staining
- CCR7
- CCS Annual meeting in Washington, DC Nov 8-10
- CCS meeting
- CCS meeting 2003
- CD 4 / CD8 double positives - revisited!
- CD10 -ve cytoplasmic mu +ve ALL?
- CD103
- CD107a and CD107b
- CD11c-PE
- CD16 on whole blood
- CD19 downregulation
- CD19 PE vs CD19 FITC
- CD229.2/ Ly9.2 /LGP100 mAb
- CD23 on T-cells
- CD25 and HLADR on Chimps
- CD27 and CD28 on permeabilized cells
- cd28 on monocytes
- CD3 > CD4 +CD8
- CD3 CD16 CD56
- CD34 enumeration
- CD34 enumeration choices
- CD34 SORTING PROBLEMS!!!
- CD4 expression question
- CD4 guidelines
- CD4/CD8 double positives...again
- CD49d costimulation
- CD56 in myelodysplasia
- CD57 antibody
- CD62L staining
- cd69
- CD69 and IFN-gamma
- CD7 - Did you get a copy?
- CD7 - many requests
- CD7 - Polysciences will Provide copies.
- CD7 - Send us stuff!!!
- CD7 - Sponsor Aprreciation
- CD7 Requests - We need an address too.....
- CD79b Antihuman McAb
- CDC ORISE position
- cell clumping or debris!!!!
- Cell Conjugation Studies
- Cell cycle analysis
- cell cycle analysis of nonsegmenting chromosomes
- cell cycle analysis of Sf9 insect cells
- cell cycle analysis on insect cells
- Cell cycle and sorting of viable cells
- Cell cycle conundrum
- Cell cycle determination on yeast
- cell cycle in bacteria
- cell cycle in cardiac myocytes
- Cell cycle in plants
- cell cycle of endothelial cells
- Cell fixation/permeabilisation
- cell line
- Cell permeabilizing
- Cell Proliferation Data
- cell separation using antibody
- Cell Surface Staining + DNA content
- Cell tracker
- cell tracker for light microscopy-summary
- Cell tracker: Thank you
- cell viability
- cell washers
- Cellquest and FACSCalibur resources
- CellQuest on OSX
- Cellquest Pro back to Cellquest
- CellQuest Pro on a G5
- Cellquest vs. Cellquest Pro
- Cellquest vs. Cellquest Pro - summary of responses
- CFP and GFP
- CFP and GFP... The Summary
- CFP/YFP FRET
- CFP/YFP FRET summary
- CFSE and immuno-phenotyping
- CFSE Detection Problems When Combined With Other Cells
- CFSE staining
- CFSE staining in MCF-7 cells.
- Channelyzer monitor, anyone?
- charcoal stripped serum
- Chicken CD69 or CD25
- Choosing the Best Fluorochrome
- Chromomycin and violet excitation
- chromosome number
- Circulating Pre-T cells
- Citing WinMidi
- Clinical Definition of a Dendritic Cell
- Clinical Flow applications using BD FACSCalibur or Coulter FC 500: References and Opinions
- Clinical studies of apoptosis
- Cloning into PCR plate - advice
- Co-authorship versus core services
- CO2 independent media
- Coherent Innova 70C Spectrum for sale
- Coherent OPSL 488-200
- COHERENT SERVICE SUPPORT
- color compensation
- Coming East
- compensation
- Compensation for JC-1 mitochondrial membrane potential
- Complement monitoring on monkey
- Components for Laser Scanning Cytometer
- Compucyte iCys demo at NIH - 2nd announcement
- Compucyte iCys demo at the NIH
- Concentration of Brefeldin A in Golgiplug?
- Conference mesure d'ions en microscopie confocale
- Confocal Calcium Flux
- Conjugation of antibodies with PE and Per-CP; mouse whole blo od staining
- Conjugation of antibodies with PE and Per-CP; mouse whole blood staining
- Constant temp refrigerator?
- Contamination issue and Sterile sorting
- Controls for Apoptosis
- converting .fcs files into text
- Cooling for Coherent EnterpriseII
- Cooling for Coherent EnterpriseII [RB-NF4GTH2]
- Cord Blood
- Correction on DRAQ5
- Correction on San Deigo Area Flow Cytometry Users' Meeting
- correction: last call: IV International ...: new Deadline June 1 0, 2003
- Cost Recovery
- Coulter Altra For Sale
- Coulter and BD Sample Preppers
- Coulter Epics XL/MCL spare parts
- Coulter Sorter for sale!
- counting events and statistical significance
- cpt CODES
- CQ File Storage
- CSF FLUID
- Csf fluids > 24 hrs old
- curve fitting analytical software
- Custom conjugation of Annexin V
- custom-made plexiglass sort chamber enclosure
- Customized Tetramer
- Cy5 and APC?
- Cy5 and APC? [ACA-E9C7V5]
- Cy5 and APC? [DC-MME4RN4]
- Cy7 Reagents
- Cy7-PE Reagent Stability
- Cy7PE
- cyclin D1
- Cyt -C assay
- Cytokine Bead array
- Cytokine Bead array and transfer of files
- Cytokine Bead Array update
- Cytokine detection kits: Luminex vs CBA
- Cytokine flow cytomtery - live vs. dead cells
- Cytomation flow analysis software
- Cytomation MoFlo Analyzer for sell.
- Cytometer for sale
- CYTOMETRY 2nd and 3rd Edition
- Cytometry job in Melbourne, Australia
- CytoServ
- Dako Instrument and FloMax
- DAKO Medimachine
- DAPI
- Dapi concentration
- Dapi concentration (with violet laser)
- Dapi concentration [SM-IVHXIW3]
- DAPI solution
- Dartmouth Flow Coiurse
- Dartmouth Flow Course
- Data Analysis of Apoptotic Cells
- data files
- Day 2 Report - Cytomics Congress
- Day 3 - Cytomics Congress, Wales
- DC collaboration
- DCFD assays on stimulated PMN
- DCFDA staining for ROS
- dead cells in sort
- Deadline for EMBO practical Course Berlin
- debris or not debris
- Detaching cells off TC plate after fixation
- Detecting D+ cells
- Detection issues
- Detection of eosinophils in mouse BAL...
- Determination of serotinin in human platelets. Request for protocol
- Determination of viability of progenitor cells using flow cytometry
- dexamethasone-FITC
- Diagonal Skewing
- Dick Mitchell
- Dick Mitchell - In Memoriam
- Dick Mitchelll is alive in our memory
- Differentiating single cells from clumps
- Difficult monoclonal to conjugate - request for suggestions
- Digital compensation and MESF simultaneously
- Digital electronics
- Digital hoohaw
- DimerX problems
- direct antibody staining prior to incubation at 37 degrees C
- Direct TUNEL
- Disaggregating bacterial flocs
- Discounts on Practical Flow Cytometry, 4th Edition
- Discussion Summaries
- distinguish human tissue from mouse tissue?
- distinguishing extracellular from intracellular bacteria
- DiVa - events on the axis ?
- DiVa and FlowJo issue
- DiVa regions & sorting
- DNA analysis
- DNA Course offered at City of Hope
- DNA dyes for viable cells
- DNA Index on Paraffin Embedded Sections.
- DNA methylation by flow
- DNA Procedure
- DNA-Staining combined with Annexin V?
- doing flow on skin cells
- doublet cell discrimination - history
- doublet discrimination
- doublet discriminator
- doublet test particles
- Doubts about ModFit.
- DQ-OVA
- DRAQ5
- Drosophila sorting and viability
- Drosophila sperm
- DsRed
- DsRed and cycle
- DsRed extraction in ethanol fixation
- DsRed in 293 cells
- DT-40 chicken B-cell markers
- DX-5 mystery
- e-mail address from R.Hicks (FCSPress)
- E-mail problems
- Early Apoptosis
- EBV by flow
- education, Flow Cytometry wokshop Fall 2003/Spring 2004
- Effective fixatives and validation (Was: Sorting live human lymphocytes) [HS-GWG6QA3]
- eGFP in FL3
- Email address
- EMBO Practical Course in Berlin
- EMBO/ELMI Workshop
- endothelial apoptosis in tissue
- eosinophil and neutrophil markers
- Eosinophil autofluorescence
- Eosinophils and Basophils
- EPICS - Noise/Discriminator Setting
- EPICS V
- Epithelial Apopotosis Quantification
- EpoR mab
- Equine cd4
- erythrocyte autofluorescence
- erythrocyte death?
- ES cell sorting and viability
- European Summerschool Immunological Techniques
- European Summerschool Immunological Techniques, 1. Announcement
- European Summerschool Immunological Techniques, Hungary, 2nd Announcement
- Event accounting on FACSCalibur
- Events on the axis
- everyone that can help
- Experience
- experience with sorting cardiomyocytes
- Extracting data from FACS measurements
- facility director position open
- FACS - Mast cells
- Facs Aria machine
- Facs Aria machine - mistype
- FACS data standards for cross-platform analysis
- FACS on lungs
- Facs sorting fixed and live cells
- FACS vs MACS
- FACS work on the Sputum
- FACSAria
- FACSAria Field Experience
- FACSArray
- FACSCalibur for Sale
- FACSCalibur Instability
- facscan connection help
- facscan for sale
- FACSDiva for sale
- facsimile: FREE flow cytometry software updated
- facsimile: FREE flow software updated 14 Aug. 2003
- Facstar Plus for sale
- Facstar Plus O-scope
- FACStarPlus - offer
- FACSVantage for sale
- Factor of 10 error
- fantastic Job
- farnesylated GFP
- fas induced apoptosis
- Fastimmune
- FDC-P1
- Feline Il-6
- Ferreting out T cells
- fibroblast and chondrocyte markers?
- fibroblasts
- FileGuard
- Final call - NIH Union Biometrica demo
- FITC labelled bacteria
- FITC soluble in chloroform?
- Fixed cells and PS
- FL1-H/FL2-H RATIO IN CELLQuest
- FL1-H/FL2-H RATIO IN CELLQuest : Other software options...
- FloJo comments
- FloJo Templates creation/use
- Flow Analyzer
- flow cell dirty and sorting
- Flow Course
- Flow cytometry -Cell quest pro
- Flow Cytometry Course
- Flow Cytometry Courses
- Flow Cytometry Job opening in New England
- FloW cytometry mailing list / Flow sorting of pancreatic A and B cells
- Flow Cytometry Position Available
- Flow Cytometry Position in Cambridge, UK
- Flow Cytometry position in coastal Maine
- Flow cytometry statement
- Flow Cytometry Technlogist Position in Beautiful New England
- flow Cytometry Training in NYC Jan. 21-23
- Flow Explorer has been updated to version 4.0
- flow for marine shellfish cells
- flow of microbiology
- Flow Software
- Flow sorting of pancreatic A and B cells
- Flow workshop at Wittenberg University
- flow-fish for telomere length
- flow/research job opportunity
- Flowrap beads
- Fluo-3 loading in Platelets
- Fluo-3 loading in Platelets; refluxing of sheath fluid
- Fluorescence COMPENSATION Workshop in San Francisco
- fluorescent beads for measuring rare populations.
- fluorescent oligosaccharide probes
- Fluorochrome conjugated ZAP70
- Fluorochrome labeled inhibitors of apoptotic proteases
- Fluorochrome-labeled inhibitors to detect activation of caspases during apoptosis
- Fluorophore question
- FOLLOWUP: non-mercury antimicrobial agents
- Followup: Two fluorophore questions
- for email archive
- For Sale: MoFlo Electronics Rack & Computer
- Free software - FCS Express
- free software for software compensation ?
- Free software?
- Free WinList Update Available
- Fresh Vs Old parformaldehyde
- FRET by Flow and Fluorochromes
- FSC-SSC dot plot of IgM-antibody loaded cells ?
- Fwd: [Plant cell suspension prep]
- Fwd: Circulating Pre-T cells
- Fwd: Guava information
- Fwd: Immunophenotyping of lymphocytes from frozen mouse blood
- Fwd: UV-laser LSR II
- G0 and G1 in non human primates
- G1/G0 discrimination options
- G5 Upgrade
- gain in fluorescence
- gamma delta T cells
- gamma IFN assay
- Garage / Lab Sale
- getting rid of remaining fluorescence
- GFP
- GFP and Bacteria
- GFP and DsRed
- GFP and PI
- GFP expression queries
- GFP expression statistics on log scale histograms
- GFP Fluorescence Intensity (FI)
- GFP lifetime in cells
- GFP, intracellular marker(PE) and HO for DNA
- GFP/YFP
- GLIIFCA Oct 3-5 reminder
- glutathion level
- Go/G1 discrimination options
- granules from eo and neutrophils
- granulocyte maturation
- Green viability stain for Bone marrow and Spleen, B cells
- GRHR-receptor
- Guava information
- Guinea Pig Antibody
- Gunk disaggregation
- HaCat cells
- Hamamatsu 957-08 (or R928)
- Hamster and mouse antibody cross reactivity
- Happy Holidays and a New Year's Proposal
- Happy Holidays and a New Year's Proposal; Response
- Happy thanksgiving everyone!!!!
- hard drive for data storage.
- Harmful FITC breakdown products ?
- Heat inactivation of IgG as an Fc block
- HELP
- help from anyone that can
- Help in protein extraction
- help please....Ross River Virus
- help prtocoll for cell lysate for CD4 work
- Help us on PMT settings on the Aria
- Help us on PMT settings on the Aria and "Amen" by Joe Trotter
- help us to choose a flow cytometer
- help with corrupted FCS files
- Help with running a new moflo
- Help!
- Hematopathologist looking for position
- Hepa masks
- heparin induced thrombocytopenia
- High content Analysis meeting
- High molecular weight DNA from mammalian chromosome sorting?
- high Reynolds number flow [JPR-IJA55E2]
- High Thru Put Sampler Manufacturer?
- high thru put sampler?
- Histogram analysis
- HIV and light chain
- HIV CTLs phenotype
- HLA (including flow) technologist
- Hoechst 33342 staining mouse embryos
- Hoechst 33342 staining of mouse embryos
- Hoechst 34580 vs 33258, 33342
- Hoechst and adherent cells
- Hoechst SP resolved
- holes in cells
- Hourly Charges
- how to answer reviewers
- how to service a Coherent heat exchanger?
- Howard's book - better discount....
- human alpha interferon
- Human Cytome Project
- Human IL-18
- Humen brain cells
- hVEGF receptor antibodies
- Hydra needed
- hygiene issues
- i/c caspase substrates
- IC IL4 Detection: Summary
- IDENTIFICATION of researchers working on drugs which affect apoptosis via flow cytometry and confocal microscopy/Urgent
- IFCC Flow Cytometry Course and Symposium
- IFN-g fluorochrome
- IgE on murine B cells
- IL-10 in flow cytometry
- IL4 intracellular staining
- ImageStream 100
- Immunodificincey case
- Immunophenotyping of frozen spleen tissue for Flow analysis
- Immunophenotyping of lymphocytes from frozen mouse blood
- Immunophenotyping of thalassemia pateints
- Immunophenotyping Rat spleen tissue
- Immunophenotyping spleen tissue
- Immunophenotyping spleen tissue from nude mice
- Immunotech CD117
- Immuophenotyping spleen tissue
- Impact on sheath on cell measurements
- Importance of Na Azide?
- in vivo apoptosis
- Inexpensive CD4 count options for Tanzania
- Infinity Air Cooled self contained outdoor chiller 20kW
- Influence of Immunsuppressiva on FACS XM results
- Info Reqd: Normal Range
- info request regd..
- Innovation Award 2003 for CyFlow and CyLab Technology
- instructional cytometry samples for students?
- interesting book
- Interesting Lymphoma Case
- Interfering Condition in Flow Cytometry Assays
- Intracellular caspase detection
- intracellular cytokines
- intracellular fret
- intracellular IFNg Ab staining
- intracellular IFNg Ab staining by conc.
- Intracellular IL-3 and/or GM-CSF?
- intracellular pH
- Intracellular Staining of Eosinophils
- intracellular staining of mouse dendritic cells
- Intrarcellular staining on thawed lymphocytes
- Invitrogen purchase of Molecular Probes
- IRAQ & GULF REGION: HEALTH-RELATED ISSUES/CONFERENCE
- Is DRAQ5 Fluorescence Affected by Bromodeoxyuridine?
- ISAC Tutorials
- ISAC XXII Workshops
- ISHAGE procedure for mice bone marrow
- ISHAGE procedure for mice bone marrow?
- isolation of BAL macrophages
- Isoton II on BD Vantage
- IV International School on Antigen-specific T Cell Cytometry
- J P Robinson' s Flow cytometry Slides.
- January MetroFlow
- Job advert.
- Job Fair at The Boston Users Group for Cytometry Compensation Meeting; 10 September, 2003
- job opening
- Job Opening - Manager/Operator
- Job openings
- Job openings - Immunology technicians
- Job openings still available
- Job posting
- Job posting in New York City
- job, Dir of Flow Cytometry
- K2 PLASTIC VS K3 GLASS EDTA BLOOD COLLECTION TUES
- keeping cells in suspension
- Keeping the cleanse clean in an XL
- Ki-67
- Ki-67 summary
- Kolmogorov-Smirnov
- Kolmogorov-Smirnov (Is there a better solution?)
- kupffer cells
- L/K/20 PerCp-Cy5.5
- Lab Equipment
- Label query
- Labeled aCD23 that blocks IgE binding
- Labeling Cryptosporidium
- Labeling kelp spores
- Labeling kelp spores [BJE-LK96DQ]
- Labeling w/ Quantum Dot
- lac z staining
- lacZ B-Galactosidase by flow
- Lambda/Kappa
- largest cell diameter to be sorted on a FACSVantage
- largest cell diameter to sort on a BD FACSVantage
- laser beam spot size.
- Laser Cooling, LP5 vs. LP20/40--- summary of responses
- laser service contracts
- last call: IV International School on Antigen-specific T Cell Cyt ometry
- Last Message on CD7
- Lazy Questions, was malignant plasma cell detection...
- Lifetime determination using FACS
- liposome transfection and DNA
- Liver cell isolation for flow cytometry
- Liver cells.
- liver marker for histichemistry staining
- London (UK) Immuno meeting. FEB 17
- Long term cultures and autofluorescence
- Looking for a good RNase that is DDNase free
- Looking for a used FACSCalibur
- Looking for EPICS pinch valve
- Looking for EPICS PMTs
- looking for flow cells
- Looking for Gwyneth Olson
- Looking for receptor vendor (IFNAR1 and IFNAR2)
- Looking for your help
- loose lens, anyone
- Losing HPAECs during prep
- LSC Info Needed
- LSR II
- LSRII virus protection
- luekemia case
- Luminex and infected samples
- Luminex in Chicago?
- Luminex system
- Lymph node preparation for analysis
- LYMPHOCYTE ENUMERATION
- LymphoSum in HIV/AIDS
- MAB to Human TCR V Beta Receptors
- mAbs Titration
- Mac Log in summary
- Mac Login Program for OS 10.2
- MacOS 9 and CellQuest problem solved
- Macrophage activation in vitro
- malignant plasma cell detection by flow cytometry
- marker
- Marsupial antibodies
- Mast cells and FSC
- max particle size on FacsCaliber
- means/%+ reply
- Measure of cell division in GFP cells
- Medicare Contractors
- Medicare Reimbursement for Flow Cytometry
- MEDIMACHINE
- Meeting for laboratory hematologists
- Megakaryocytes
- Melanin and light scatter
- Members of this list
- Membrane potential dye DIIC5
- Methods in Cytokine Biology
- MetroFlow announcement
- MetroFlow Meeting
- MetroFlow meeting announcement
- MetroFlow meeting reminder
- MetroFlow Reminder
- MFI calculation urgent
- MFI calculations
- MFI Calculations: Okay to subtract the unstain'd cells' calculated value from samples?
- MFI data
- MHC Class II
- Microarray motifs from tissue-derived cells
- Microbeads as internal standard
- microparticles
- microscope for live cell imaging
- minimum event number needed to get valid MFI
- Missed Opportunity for a Pun
- Missing Splenocytes on a MoFlo Sort
- mitotic index
- Mitotracker red
- Modfit Lt
- ModFit LT 3.1 Service Pack 1 now available for download
- MoFlo compressor
- MoFlo or parts
- MoFlo Safety Hood
- monobromobimane
- Monoclonal sIg reagents
- Monocyte autofluorescence
- Monocyte marker
- Monocyte marker and comprehensive compendia
- Monocytes Disappear after Bead Treatment
- More nylon screen suppliers
- Mouse Bone Marrow Flow
- mouse dendritic cell marker
- mouse lymphocyte activation marker
- Mouse reticulocytes
- Mouse sperm and nude female mice
- mRNA loss during cell fixation
- multiple biotin/streptavidin conjugates in same prep?
- Murine bone marrow staining for AFP
- murine leukemia cells
- Murine Megakaryocyte Isolation
- murine megakaryocytes
- Myeloma thread
- NCI-Frederick and CCC Cytometry Meeting
- NCI-Frederick and CCC Cytometry users group meeting
- Need advices to buy a cytometer
- Need advices to buy a cytometer: summary
- Need information on how to label LDL with BODIPY-FL
- New CYtometer
- New Plain Text ASCII Conversion Documentation for MFI Software
- New Topic by Matt Ottenberg - "Dick Mitchell - In Memoriam"
- NIH Flow User Group meeting
- NIH iCys demo - associated conferences
- NK-T cells
- non-mercury antimicrobial agent needed
- non-specific binding of streptavidin (intracellular)
- Non-specific binding of streptavidin EXTRA CELLULAR( PMNs)?
- Normal ranges for naive and memory T cell
- NPE Quanta 488
- nRBC in Thalassemia
- nylon mesh pore size
- nylon screen suppliers
- Occupational ratio
- Of Mice and Men!!
- OKT-3
- old FACScan and WINMDI
- old FACScan and WINMDI [DEB-WAICCJ2]
- Oligo-Flow?
- On-axis events on FACSDiva
- Oncotech DiSC Assay by Flow cytometry
- online articles
- Open NIH demo - ICyt Lyt-200C laser
- Open position
- Optimising an Omnichrome UV for Indo-1
- Ortho Spectrum Pictures
- oxidative burst - to pool or not to pool, that is the question
- Oxidative Burst for craig@upmc.edu
- oxyDNA assay
- oxygen species in biological membranes
- P3 sorting
- Paint-a-gate
- Parallel Testing and Antibody Titration
- PCR on intracellular stained cells
- PE and alcohol
- PE-Cy5 Issues on FACSCalibur Machines
- PE-Cy7 FRET
- PE-Cy7 vs APC-Cy7
- Pediatric oxidative burst
- Peds ranges, HIT testing
- PerCP
- permeabilization control- annexin
- Permeabilizing of 'subjective' populations
- permeabliliztion controls
- Permeablizing and staining bacteria
- peroxisomes
- PhD Enquiry
- Phenotype Question
- phenotype question Update
- Photo-bleaching PE
- PI and BrdU
- PI staining for Apoptosis
- PI viability study with the presence of NRBCs
- PI Viability vs MTS
- PI vs 7AAD
- PicoGreen P7581 Dye chemistry question
- Plant flow cytometry
- plant protoplasts
- Plasma cells
- Platelet Activation Markers
- Please accept my warmest thanks for your helpful answers
- please help - hygene issue
- please help - hygiene issue
- Please post PD for Flow positions (2) on Flow website
- Plt intracellular calcium
- PNH Panels
- Polymyxin B !
- pore size of nylon mesh
- Position
- position available
- Position available - London UK
- Position open
- Position open at NIH
- Position still open
- Positions Available
- Positions available: Supervisor, Technologist
- Positions Open
- Positive dual staining
- Possible contamination
- Postdoctoral Position
- Postdoctoral Position at NIH
- Postdoctoral Research Fellow in Cytogenetics
- Posting Questions
- PR and ER by flow
- Practical Flow Cytometry, 4th Edition - Birth Announcement
- Pre osteoclasts and osteoclasts by FACS
- Pre-GLIIFCA DNA course
- Pre-GLIIFCA DNA course Deadline...
- pregnancy and flow work
- Preparation of (mouse) lymph nodes
- Preparation of cell suspension for Flow analysis from solid tumors
- Primary versus cell line
- probes for mitochondria
- Problem with adherence of Macs
- problem with CFSE staining
- Problems with 4 colour staining
- Problems with a seasick Facscalibur
- Processing Peripheral Blood and Bone Marrow Samples
- Proposed Workshop on Mulitcolor Compensation
- protocol for adherent cells
- Protocol for stimulation/activation of normal human B-lymphocytes required....
- Protocol to permit culture of viable cells, etc.
- Provider of CMV antigen
- PSR sensitivity
- Puzzling PI staining with fluorescently labelled caspase inhi bitor
- Puzzling PI staining with fluorescently labelled caspase inhibitor
- Quality Control in the Flow Lab
- quantitation of malignant cells in lymphoma patients
- quantium beads
- Quantum dot analysis
- quantum dots
- Quenching Autoflourescence
- quenching GFP fluorescence
- R636-10 PMT
- Rabbit and sheep cytokines
- Rabbit anti-goat antibodies
- rabbit anti-PE
- Rabbit TCR
- RANKL monoclonal antibodies
- Rat antibodies
- Rat Beta Cells
- Rat CD3 expression
- rat myocyte stain/sort
- rat RBC sorting
- rat-human cross-reactivity
- RBC Viability
- RE : BD FACSCanto
- re azide
- Re cell cycle analysis of sf9 cells
- re CellQuest on OSX
- Re- Behshad : everyone that can help for RBC....
- Re;FACSAria
- Re;FACSAria (UV lasers)
- reading DIVA FCS files with cellquest
- Recommendation IFNg/IL-4 intracellular staining
- Recover of lost FCS data file
- Red-excited alexa dyes
- regarding anti-human CD14 and TLR4 antibodies
- Registration Deadline for Flow Courses at UC Davis
- Registration for Boston Users Group for Cytometry Compensation Meeting
- Registration for Boston Users Group for Cytometry Complex Multicolor Flow Cytometry and Compensation Meeting
- replacement for old facscan
- replacing APC by Cy5PE and Cy5PE by Cy5.5PE
- reply to FACS on lungs
- reporter genes in cytometry
- repost: RNA isolation after BrdU labeling
- Requesting titles of few interesting research areas for the presentation.
- RES: cd69
- Research Assistant Post
- RESEARCH TRIANGLE CYTOMETRY ASSOCIATION 09-APRIL-2003 10:00 AM
- Research Triangle Cytometry Association 09-Dec-2003 09:00-12:00
- RESEARCH TRIANGLE CYTOMETRY ASSOCIATION 20-MAR-2003 09:30
- Research Triangle Cytometry Association 23 Jul 2003 09:00
- RESEARCH TRIANGLE CYTOMETRY ASSOCIATION 26-JUN-03 10AM
- Research Triangle Cytometry Association 30-Jan-2003
- Research Triangle Cytometry Association Wed, 06-Aug-03, 10:00
- Respiratory burst and dihydrorhodamine
- Retention of fluorescent antibody after culture
- Retention of fluorescent antibody after culture-Summary
- Rhesus monkey CD55, CD59 Antibody
- rhodamine & mitochondria
- rhodamine assay in brain
- Rituximab and anti-CD20 staining
- RNA isolation after BrDU labeling?
- RNase contaminations?
- Rodent's granulocyte seperation.
- ROS production
- RTCA Announcement
- Réf. : EBV by flow
- Réf. : Free software?
- Réf. : Heat inactivation of IgG as an Fc block
- Réf. : MFI Calculations: Okay to subtract the unstain'd cells' calculated value from samples?
- Réf. : PNH Panels
- Réf. : Two question of basic methodology from a beginner
- Salary survey
- Sale for "FACS Calibur" and "Elispot reader"
- sale of a confocal system
- Sample tubing
- Sample tubing source
- San Dieago Area Users' Group Meeting
- SAN DIEGO AREA FLOW CYTOMETRY MEETING
- San Diego Flow Users Group Meeting
- San Diego Flow Users Meeting
- SCID
- SCIDS
- Scientific Research Officer/Flow operator post
- Search PBP2a antibody for MRSA
- Seeking ADB Cellquest dongle
- Seeking BMW "ignition dongle"
- Seeking clinical comments
- Seeking Coulter FC500 users
- Seeking FGFR3 antibody alternatives
- Seeking software for Wallac Microbeta
- Selecting cells to use for screening
- sensitivity of ELISPOT vs. intracellular cytokine staining
- sensitivity of flow cytometric analysis
- Separate two daughter cells after cell division
- Separating two populations using mAbs against weakly expressed populations
- SF Bay Area COMPENSATION WORKSHOP
- Shapiro's 4th Ed "Practical Flow Cytometry"
- Shapiro's 4th Edition "Practical Flow Cytometry"
- Sheath at nozzle pressure gauge?
- sheath pressure regulation
- side population cells and Hoechst 33258
- SIG grants
- Size difference: Monocytes vs Activated Macs ?
- Small particles
- snarf loading
- snarf loading of cells
- Software for MacOSX and PC (WEASEL)
- software question
- Software to identify or map T cell epitopes
- Software to overlay dot plots for analysis
- Solid state UV laser
- Solid state UV laser [2]
- Soluble CD4
- Some suveys coming.. This one calibration, standardization and quantification
- somewhat off topic - ACK media lysis on apheresed blood
- Sorry for the double posting
- Sorry Howard
- Sort operator in Chicago
- Sort Watch
- Sort Watch Responses
- Sorter Hours Accounting
- sorter locations?
- sorter operator position available - San Francisco CA
- Sorter Specialist needed
- Sorting and FACScalibur
- sorting bacteria based on morphology
- Sorting drosophila cell lines
- sorting facility and
- sorting GFP+ E.coli
- Sorting HEK 293 and establishing clonal cell lines
- Sorting Isolated Cardiac Myocytes
- Sorting live cytokine producing cells
- Sorting live human lymphocytes
- Sorting live human lymphocytes - so far
- Sorting live human lymphocytes [JPR-NGT5823]
- Sorting live human lymphocytes: Will be on CD7
- sorting lymphocytes
- Sorting neurons
- sorting pigmented cells
- sorting plasmids
- sorting spleen DC
- Sorting synaptosomes
- Sorting tumor cells
- Source of CD73 antibody
- SP and 407
- SP cells with a 405
- SpamArrest Warning
- Specific gravities
- Spectra of fluoroquinolones
- SpectrAlign question
- Sperm and PCR
- Sperm cell surface receptors
- sperm staining
- Spherotech 8-peak on a Vantage
- Spherotech 8-peak on a Vantage [RH-R2N8H42]
- SPIE BiOS 2004
- spurious data
- squirrel monkey Abs
- squirrel monkeys
- Stabilcyte
- stabilization of IgM
- staining suggestions?
- Standardization of immunophenotyping
- Staphylococcal Enterotoxin B
- Statistical robustness
- stats question
- stem cell markers in the rat
- Stem cells through FACS without Ab...possible?
- Sterile sorting (again)
- sticky problem with MCF-7 cells
- Strange CD4 FITC results
- Strange medullar population
- Strange response observed from Fluo-3 and calcium flux
- sturdy B-cell marker
- sub-saturating antibody amounts
- subtraction of isotype control
- Suggest a DNA stain
- Suggested readings
- Suggestions for naming of digital files
- Summaries are now posted on website
- Summarize "Free software"
- Summary of GFP/YFP Responses (13)
- summary of Isoton II on BD Vantage
- Summary of Responses - Boston Users Group for Cytometry Compensation Workshop
- summary of responses - cell cycle of endothelial cells
- Summary of responses about FACSAria
- Summary of responses concerning DakoCytomation's SUMMIT software
- summary of responses-conjugation of antibodies with PE....
- Summary on "HIV and light chain"
- Summary-Stabilization of IgM
- Summary/Use of the Term "Purification" in Sorting/Publication
- Survey - Report
- Survey problem fixed...SORRY
- Survey Request for Practicum
- Survey: Calibration closing 12 MD 22 December
- SV: FL1-H/FL2-H RATIO IN CELLQuest
- SV: Fluo-3 loading in Platelets
- T-LGL Leukaemia
- tagged-peptide detection using FACS
- tdt positive control
- Tech needed
- Technical staffing levels
- Technician Position
- Technological advances: Rhyme or reason?
- Technologist Position Available
- TER119-PE vs TER119-APC
- testis cell sorting
- tetramer synthesis
- TH1 Superantigen
- TH1 superantigen and SEB
- Thank you
- Thank you all!
- Thanks a lot for your advices! Link to StuffItExpander download for free!
- Thanks all
- Thanks for the feedback
- Thanks for your help
- Thawing bone marrow MNCs
- The Isotype question!
- Thoug hts on buying a FACSarray
- THP-1 cell line
- tlr4
- To-PRO-3 Viablity Stain
- to-pro3 dimmed ?
- To-Pro3 DNA histogram
- TOPRO-3
- tracking migrating monocytes by DNA dye ??
- transfection marker
- treshold on FL4
- Triple staining (FITC, PE, PerCp) lymphocytes by mono-color mAb.
- Triple staining of polymorphonuclear cells
- Trouble with opening CellQuest files in WinList
- TRYPAN BLUE
- trypsinization and cell surface proteins
- TTP LabTech Acumen Explorer demo in D.C. Metro area
- Tubes/Known Number of Beads
- TUNEL&detecting of FasL
- Two fluorophore questions
- Two question of basic methodology from a beginner
- tyramide signal amplification
- UK viability dye?
- Unexpected difference in number of positive cells
- Union Biometrica sorter demo at NIH
- Unusual viability/non viability pattern
- Update on Boston Users Group for Cytometry Compensation Meeting
- Upper Midwest Flow Cytometry Users Group Meeting
- URGENT HELP NEEDED
- USE OF CD45 AS ALTERNATIVE MARKER
- Use of Cy5 with bacteria on FACScalibur
- use of JC-1
- Use of the Term "Purification" in Sorting/Publication
- Use of viability dyes when sorting
- Used FACScan/FACSCalibur
- Used Instrumentation for sale
- Using DI Instead of Isoton
- using rhodamine123
- using TO-PRO-3 for cell cycle analysis
- UV dyes
- UV laser and SP Hoechst problems
- UV laser or violet diode
- UvV flourochromes
- vaccines
- Validation of viability determination by flow cytometry
- Very strange case
- Viability after sorting
- Viability of human mast cells
- viability protocol
- violet laser on LSR II
- Waldenstrom
- Waldnstrom
- Wanted: Shipping Crates for FACSVantage
- Weapons of mass detection
- What does hydroethidine react with?
- What does hydroethidine react with? Reply
- What is this population?
- Where is Ken Castleman
- Who has online access this article?
- Who has online access to the requested articles?
- whole blood cultures
- Whole Blood Stimulation
- Width/area signals on the FL3 and height-signals on the FL4
- Wiley 15% Discount on PFC4
- Windows time logging program
- WinMDI 'empty' is displayed on both X-and Y-axis
- WinMDI under windows xp
- WinMDI under windows xp - works fine for us
- WinMDI under windows xp [JMB-ESSKCN4]
- WinMDI under windows xp and CYTOMATION
- Workshop Announcement
- World Patent Application "Cell Enumeration", dual platform method
- Yeast Cell Cycle?
- yeast contamination
- YFP - GFP discrimination on BD LSR II
- YFP-GFP
- You know you are a flow cytometry junkie when.....
- Young Investigator award
- Zap -70
- Zap 70 conjugate
- zap-70
- ZAP-70 antibody
- Zenon combined with MACS
- zenon labeled rabbit Ab
- zenon labeling
- Zenon Labelling
- ZsGreen expressing cells with PI staining
Last message date: Wed Dec 31 2003 - 14:18:01 EST
Archived on: Thu Jan 01 2004 - 17:44:07 EST
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