granules from eo and neutrophils

From: Kim, YaeJean (NIH/NIAID) (YJKIM@niaid.nih.gov)
Date: Wed Dec 24 2003 - 10:48:21 EST

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Hi. I have a question about granules from neutrophils or eosinophils. Does
the flow can detect the granules released from these cells?(maybe this is
the question regarding the lowest limit of size for particle detection by
flow) I have purified neutrophils and eosinophils run by flow and some tubes
activated with antigens I see little debries like group of particle with
decreased FSC.

Could they be granules? IF so, can I somehow stain the granules and see
them?

Thank you for any advices and happy holidays.

YaeJean 

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