From: Mara.Rocchi@mri.sari.ac.uk
Date: Wed Dec 24 2003 - 05:17:15 EST
Hi Liza, Have a look at this: J Immunol. 1995 May 1;154(9):4294-301. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+CD27- lymphocyte subpopulation. Elson LH, Nutman TB, Metcalfe DD, Prussin C. They describe a protocol for cryopreservation. good luck Mara ******************************* Dr. Mara Rocchi Ph.D. Flow Cytometer Manager Moredun Research Institute Pentland Science Park Bush Loan, Penicuik EH26 0PZ Scotland, UK Tel: +44-131-4455111 Fax: +44-131-4456111 e-mail: mara.rocchi@mri.sari.ac.uk -----Original Message----- From: lmcguire [mailto:liza.mcguire@mail.tju.edu] Sent: 23 December 2003 02:09 To: cyto-inbox Subject: Intrarcellular staining on thawed lymphocytes Hi flowers, Does anyone have experience with evaluating intracellular staining on thawed human lymphocytes? We currently stain fresh cells with Perforin but would like to be able to save the specimens received for future evaluation by cryopreserving them and later on thawing them and staining with Perforin or other cytokines. Is this achievable? What kind of problems can we expect? Has anyone done any recovery analysis, post thaw, to identify any obvious loss of cell surface epitopes? We will be staining with a couple of cell surface markers in addition to the intracellular staining. I appreciate any and all suggestions, information, guidance. Thank you for your help. <http://www.smileycentral.com/?partner=ZSzeb001> Happy Holidays <http://www.smileycentral.com/?partner=ZSzeb001> Liza McGuire Cancer Center Regulatory & Compliance Officer Thomas Jefferson University Hospital 125 South 9th Street 801 Sheridan Philadelphia, PA 19107 Phone: (215) 955-9384 Fax: (215) 955-9641 **************************************************************************** **************************************************************************** *********** The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute, disclose or copy this message and/or its attachments. <http://www.smileycentral.com/?partner=ZSzeb005> The information contained in this e-mail message and any files transmitted with it is confidential and may be legally privileged. It is intended only for the addressee and others authorised to receive it. If you are not the intended recipient or the person responsible for delivering the message to the intended recipient you are advised that you have received the e-mail in error and that any disclosure, copying, distribution or action taken in reliance on the contents of the e-mail and its attachments is strictly prohibited and may be unlawful. Please advise the sender immediately by replying to this e-mail and delete it and all copies from your system.
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