From: lmcguire (liza.mcguire@mail.tju.edu)
Date: Mon Dec 22 2003 - 21:09:27 EST
Hi flowers, Does anyone have experience with evaluating intracellular staining on thawed human lymphocytes? We currently stain fresh cells with Perforin but would like to be able to save the specimens received for future evaluation by cryopreserving them and later on thawing them and staining with Perforin or other cytokines. Is this achievable? What kind of problems can we expect? Has anyone done any recovery analysis, post thaw, to identify any obvious loss of cell surface epitopes? We will be staining with a couple of cell surface markers in addition to the intracellular staining. I appreciate any and all suggestions, information, guidance. Thank you for your help. Liza McGuire Cancer Center Regulatory & Compliance Officer Thomas Jefferson University Hospital 125 South 9th Street 801 Sheridan Philadelphia, PA 19107 Phone: (215) 955-9384 Fax: (215) 955-9641 ******************************************************************************************************************************************************************* The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute, disclose or copy this message and/or its attachments.
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