From: Andy Johnson (andy@brc.ubc.ca)
Date: Fri Dec 19 2003 - 15:01:29 EST
Hello Can I just ask if anybody else is having trouble converting DiVa files into similar FlowJo files ? There appears to be no problem when files are not compensated in DiVa. The problem occurs when DiVa compensated files are read in FlowJo resulting in "massive" over compensation of the data. Unfortunately we have got little help from FlowJo other than try their new version...which we have with similar results. We are looking at 8 colours plus, so is it just that the compensation matrix of the DiVa and flowJo clash ? We could save uncompensated data in DiVa and look at that in FlowJo...but for the price you pay, this should not have to be the case ? Plus we are saving large files of rare events, so this would remove a large portion of our sorting sample. Any suggestions ? Andy Merry Christmas -- Andy Johnson FACS Facility Manager Biomedical Research Centre UBC Vancouver 604-822-7838
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:44:06 EST
