From: David Coder (d_coder@MSN.com)
Date: Thu Dec 18 2003 - 10:41:39 EST
Here's a reference to a cytokine capture method combining the identification of activated cells and dead cells along with phenotyping that we published last year. It could be easily used with cell sorting with any dual laser system. Mathioudakis G, Coder D, Fefer A. Multiparameter cytokine-specific affinity matrix assay for the determination of frequencies and phenotype of antigen-reactive T cells. J Immunol Methods. 2002 Feb 1;260(1-2):37-42. ================ David M Coder, Ph.D. Consultant in Cytometry Seattle WA Cell/Msg: 206 499 3446 Email: d_coder@msn.com _____ From: Phil Marder [mailto:mr_redram@eudoramail.com] Sent: Wednesday, December 17, 2003 4:47 AM To: cyto-inbox Subject: Re: Sorting live cytokine producing cells Perhaps the Miltenyi cytokine "catch" system could provide the solution. Check out their cytokine secretion products here: http://www.miltenyibiotec.com <http://www.miltenyi.com> -Phil Marder -- --------- Original Message --------- DATE: Tue, 16 Dec 2003 10:56:48 From: To: cyto-inbox Cc: I need to be able to sort live IL17 producing T cells. Does anyone have any ideas on how to achieve this? I thought of the cytokine capture assay but I could not find anything for IL17. Any suggestions? Thanks- Joanne Lannigan, MS Director, Flow Cytometry Core Facility University of Virginia Jordan Hall, Room 7067 P.O. Box 800734 Charlottesville, VA 22908-0734 Office: 434-924-0274 Lab: 434-243-2695 Fax: 434-982-1071 email: joannelannigan@virginia.edu
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