Re: FACS on lungs

From: janet dow (jldow@unity.ncsu.edu)
Date: Mon Dec 15 2003 - 15:49:46 EST

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Dear Philip:

I have done flow on Cat BAL(Bronchial Alvelar Lavages) and had great 
difficulty with autoflourescence with the 488 nm laser but were able 
to use APC labeled ab using a 633nm laser.

hope this helps.

Janet Dow



At 10:53 AM -0500 12/15/03, Barren, Phil wrote:
>FLOWers
>
>	Is anyone currently ( or in the past) done any Flow Cytometry 
>on lung tissue (
>Mouse, mechanical dissociation)??
>
>	Infiltrating immune cells and changes in response to 
>challenge and treatment
>
>We are approaching this work for one of our models, and I was 
>wondering if any one has
>any experience and pointers.
>
>Thank you for your time
>
>Philip Barren


-- 
Janet Dow
Research Technician and Manager
Flow Cytometry Facility
North Carolina State College of Veterinary Medicine
Room C-314
Raleigh, NC 27606
(919)513-6364

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