From: Jason Hoffman (JHoffman@gnf.org)
Date: Fri Dec 05 2003 - 14:52:59 EST
Try buying a "functional grade" purified antibody (I also use serotec.com and ebiosciences.com; they are azide and carrier-free preps) and then labeling it yourself with Molecular Probes Alexa-488 Antibody labeling kit. Bests, jason ---------------------------------------------------------------- Jason Hoffman, PhD Genomics Institute of the Novartis Research Foundation Department of Protein Sciences 10675 John Jay Hopkins Drive La Jolla, California 92121 Ph: 858-332-4608 Fax: 858-812-1746 > -----Original Message----- > From: Andrew Beernink [mailto:ABeernink@novasite.com] > Sent: Thursday, December 04, 2003 4:53 PM > To: Cytometry Mailing List > Subject: Azide/Preservative-Free Ab conjugates > > > Hi guys & gals- > > Does anybody know where I might get anti-Human CD antibodies > (conjugated) WITHOUT azide or other "preservatives"? I've > already determined that the stock Ab's from B-D and > Pharmingen are influencing my functional analyses, in ways > not related to their epitopes. I'm pretty sure it's the > azide, as free sodium azide at comparable concentrations > similarly trashes my assays. > > B-D wants $3288 for 2 mg of aCD4-Alexa488, as a custom order; > they won't prepare a smaller amount. > > I've considered a) Zenon reagents, and b) dialysis. I'd > rather get an Ab that I can titrate and run with! > > Thanks for any input. > > Andrew > > > > Andrew Beernink > Research Scientist > Manager, Flow Cytometry Facility > Novasite Pharmaceuticals, Inc. > 11095 Flintkote > San Diego, CA 92121 > (858) 638-8584 > (858) 597-4943 fax > >
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