Detaching cells off TC plate after fixation

From: Albert Tai (acktai@exelixis.com)
Date: Fri Dec 20 2002 - 14:09:28 EST

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Hi fellow flow-ers,

Happy Holidays !  I would like to seek some advices from you for detaching fixed cells in 96 well TC plate(by formaldehyde and then permeabilized by MetOH) to run on BD multi-well autosampler.  Is it possible to lift the cells off the well after fixation ?  What would you recommend, trypsin, accutase or cell dissociation buffer to preserve surface receptor for Ab staining ?

Thanks very much in advance for your help !

Albert Tai

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