Smash and Grab

From: Michael Corey (coreym@targen.com)
Date: Fri Dec 20 2002 - 16:33:12 EST

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Hi Folks,
My first posting. I'm pretty new to FACS (most of my experience is in
enzyme assays and molecular biology). What I'm trying to do right now
is smash up some PBMCs to generate debris for use as a standard to
characterize naturally occurring debris from expansion cultures. I
tried freeze-thaw but got only aggregated material--I need a method
that solubilizes everything, so that I can FACS the debris from a
defined number of cells. What do people think?

Thanks much and happy holidays,
Michael
--
Michael J. Corey, Ph.D.
Associate Director, Assay Development
CellExSys
1100 Olive Way, Suite 100
Seattle, WA 98101
USA
Tel.: 206-521-4846
Fax: 206-521-4841
Email: coreym@targen.com

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