From: Howard Shapiro (hms@shapirolab.com)
Date: Wed Dec 18 2002 - 21:51:24 EST
Jonni Moore wrote- >A friend of mine forward some questions on the Cell-Dyn 4000..does >anyone have experince with this instrument and can you help me answer >her questions?? > >" we were evaluating the Cell-Dyn 4000 for its T cell subset counting >abilities. Well, we've got it all set up and are ready to go with >the comparing it to Simulset. Having reviewed the CDC guidelines, I >am concerned about the Cell Dyn meeting the requirements. For >instance it doesn't do the CD45/CD14 gating, and it's not clear how >one would get a gate purity or proportion measurement. Basically, >the Cell-Dyn 4000 just does the 5 part differential, with additional >activated T cell, viable leukocyte, and nRBC counting features not >found on previous Cell-Dyn versions. Then, it does a CD3/4 and CD3/8 >identification and calculates the total CD4, total CD8, and total T >cells. It does not do any CD19 or NK cell staining. Do you have any >perspective on this question? " The Cell-Dyn 4000 is essentially implementing a "two-platform" CD4 count on a single instrument; it uses the hematology tube to get the WBC and per cent lymphocytes, and the immunofluorescence tubes for CD3/CD4 and CD3/CD8. Given the relatively long gestation period, I expected Abbott to add more in the way of immunophenotyping capability to the instrument; I'm not sure why they didn't. Unless I'm mistaken, the Cell-Dyn 4000's analyses do fall within the CDC guidelines, and there are other clinical instruments, for example, the BD FACSCount, that just do CD3/CD4 and CD3/CD8. -Howard
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