From: Jonni S. Moore (moorej@mail.MED.UPENN.EDU)
Date: Tue Dec 17 2002 - 15:06:17 EST
Dear Flow Folks, A friend of mine forward some questions on the Cell-Dyn 4000..does anyone have experince with this instrument and can you help me answer her questions?? " we were evaluating the Cell-Dyn 4000 for its T cell subset counting abilities. Well, we've got it all set up and are ready to go with the comparing it to Simulset. Having reviewed the CDC guidelines, I am concerned about the Cell Dyn meeting the requirements. For instance it doesn't do the CD45/CD14 gating, and it's not clear how one would get a gate purity or proportion measurement. Basically, the Cell-Dyn 4000 just does the 5 part differential, with additional activated T cell, viable leukocyte, and nRBC counting features not found on previous Cell-Dyn versions. Then, it does a CD3/4 and CD3/8 identification and calculates the total CD4, total CD8, and total T cells. It does not do any CD19 or NK cell staining. Do you have any perspective on this question? " Thanks for any insights and happy holidays to you all! Jonni -- Jonni S. Moore, Ph.D. Associate Professor Director of the Biomolecular and Cellular Resource Center Director of Clinical Flow Cytometry Department of Pathology and Laboratory Medicine and Director, Flow Cytometry and Cell Sorting Shared Resource Abramson Cancer Center University of Pennsylvania School of Medicine 203 John Morgan Bldg. Philadelphia, PA 19104-6082 Phone: 215-898-6853 FAX: 215-898-4227
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