From: keb3@georgetown.edu
Date: Mon Dec 09 2002 - 10:06:26 EST
To whom it may concern: I am trying to set up a functional assay for MHC II expression by taking white blood cells, putting them in vitro, stimulating with SEB and measuring CD69 expression using FACS. However, I am working with rat WBC's and am having a very difficult time finding antibodies against CD69 to do FACS with. It was suggested to me to look at CD40L or CD25 but neither of those have anti-rat antibodies against them. If anyone has any suggestions on how I can get around this problem I would really appreciate it. I am trying to do FACS on these activation markers. Please let me know as soon as possible. Thank you. Karen Beagles Georgetown University
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