From: Richard Haugland (richard.haugland@probes.com)
Date: Wed Dec 04 2002 - 22:02:10 EST
Interesting question. The chemiluminescence peak of luciferin is at about 560 nm, which is a bit shorter wavelength than phycoerythrin emssion. Of course, it does not need any excitation to get the emission. But then, I don't know if the rate of emission will be fast enough to get enough photons during the transit time that you will be able to detect the signal. But then S/N should be great because all photons should be "real." I don't know what technical difficulties there may be doing sorting with the laser turned off but hope it works. Nan Jiang wrote: >Dear all: > >I am wondering if anybody ever sorted cells infected with luciferase >plasmid. Or is there a particular wavelengh we can use to detect >luciferase activity in cells and sort them based on that. > >Thanks in advance. > >Nan Jiang > >Department of Internal Medicine >Cardiology Division >UT Southwestern Medical center at Dallas >6000 Harry Hines Blvd. >Dallas, TX 75390-8573 >(214) 648-1175 >(214) 648-1181 > > >
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