From: Capocasale, Renold [CNTUS] (RCapocas@cntus.jnj.com)
Date: Tue Dec 03 2002 - 12:00:17 EST
Hello to all, I would like to sort primary splenic B cells that will have been frozen prior to sorting. The cells will be frozen for less than a week prior to sorting. In particular, should the cells be thawed and immediately sorted or rather should a recovery period be implemented and for how long? Also, has anyone tried surface staining immediately before freezing followed by sorting at a later date? Any recommendations on the nuances of this type of sorting would be much appreciated. Thanks Renold Capocasale Senior Associate Scientist Experimental Pathology/ Cell Sorter Core Facility Centocor, Inc a Division of Johnson & Johnson phone # 610 - 651- 6421 fax # 610 - 651- 7363
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