From: Alice L. Givan (Alice.L.Givan@dartmouth.edu)
Date: Wed Nov 13 2002 - 16:14:12 EST
I cannot, at the moment, find the reference --- but there is a problem with trypan blue from certain manufacturers: it doesn't quench external fluorescence even at enormously high concentrations. I believe the conclusion was that it might be a minor contaminant in the trypan blue that is really doing the quenching. Anyway, I certainly used trypan blue at New England clam chowder concentrations and had trouble getting quenching. I switched to using Molecular Probes anti-fluorescein antibodies --- and got good quencing of external fluorescence (but at a price). Then, after the fact, I read the article which explained that I should have looked to a different vendor for my trypan blue. If anyone has the trypan blue reference --- perhaps they could post it to the network. Otherwise, I will keep looking until I find it. Alice Alice L. Givan Director, Englert Cell Analysis Laboratory Norris Cotton Cancer Center Dartmouth Medical School Lebanon, NH 03756 USA Tel 603-650-7661 Fax 603-650-6130 givan@dartmouth.edu
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:10 EST
