Re: use of multiple fluorescent cell tracking dyes

From: Adrian Smith (adrians@optusnet.com.au)
Date: Wed Nov 13 2002 - 17:30:32 EST

Next message: Alice L. Givan: "Re: Internalization of antibodies"
Previous message: Otten, Gillis: "FACSCalibur fluorescence sensitivity"
In reply to: Andrew Herman: "use of multiple fluorescent cell tracking dyes"
Next in thread: Jonni S. Moore: "Re: use of multiple fluorescent cell tracking dyes"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

At 10:34 AM +0000 13/11/02, Andrew Herman wrote:
>Dear Flow-ers,
>I have a friend who wishes to identify adoptively transferred
>murine cells approx. 12 hours after injection. He would like to track 2
>separately labelled, or "tagged" cell populations, staining with
>multiple cell surface markers after isolating the cells from the
>recipient, but isn't interested in cell division. Because of the
>experimental setup congenic markers wouldn't be suitable. Am I right in
>thinking that CFSE would be too bright at this timepoint?
>Any info would be greatly appreciated.
>Thanks,
>Andy
>
>----------------------
>Andrew Herman
>Department of Pathology & Microbiology
>University of Bristol



You can always use a lower concentration of CFSE.

Adrian

Next message: Alice L. Givan: "Re: Internalization of antibodies"
Previous message: Otten, Gillis: "FACSCalibur fluorescence sensitivity"
In reply to: Andrew Herman: "use of multiple fluorescent cell tracking dyes"
Next in thread: Jonni S. Moore: "Re: use of multiple fluorescent cell tracking dyes"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:10 EST