From: Alan Saluk (asaluk@scripps.edu)
Date: Wed Apr 10 2002 - 12:27:48 EST
Flowers-- What fluorochrome pairs have been successfully used for observing surface colocalization and subsequent FRET. I have had some nice results using intrinsic CFP to YFP (with 407nm excitation) in transgenic systems, but I now have a researcher who wants to evaluate surface protein colocalization in isolated subcellular components. While there are some obvious ones that come to mind (PE to Cy5--probably not the most efficient), I was curious if there is any practical experience to tap into regarding other pairings. My excitation options are 351nm, 407nm, and the remaining kryton/argon multiline options (457-647nm). See you next month in America's finest city Al
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:36 EST
