
This page is for the use of students who are taking BMS 631
2020 Notes - Location: Vet Path Research Bldg 119
This is a 2 credit course that is designed to bring a student up to date on the technology of flow cytometry. The course will be lecture based and will require students to give a short seminar at the conclusion of the semester which will be part of the final grade. The course starts with the historical discovery of the technology and will move to discussing each of the individual components - optics, electronics, fluidics, data processing and analysis. In addition, we will cover all of the current applications. At the conclusion of the course, students will have an excellent understanding of the field and will be capable of reading, interpreting and carrying out studies using this technology.
Purdue University Cytometry Laboratories
NOTE: The distribution of these materials is restricted without specific permission of Professor Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. Specifically, no permission is given to reproduce any of these materials for courses outside not-for-profit educational institutions and permission should be requested and credit included when used in this instance. We normally give permission for use of these materials in academic institutions.If you submit these to course hero, course hero has been notified that anyone submitting these lectures must be reported back to Purdue University.
Also, please note that I have added a couple of e-lectures that highlight the introduction to flow cytometry. These are in the E-Lectures option one level previous to this page.
E-lectures (You can take a look at these lectures - they are designed as introductory lectures for those will no knowledge of flow cytometry.)
Class text is "Practical Flow Cytometry", 4th ed (Shapiro) you can download a free copy here
Date | Lect # | 2020 Lectures | Lect | File (incl older lectures) | File Size | Link |
Jan 13 | 1 |
Introduction, history & basic flow principles (Pt 1) (2019) | JPR | (Automated PPT) 2016 Lecture | updated 2020 | |
Jan 27 | 2 | The Who and Why of Flow Cytometry: History Cont. (Pt 2) (2020 Version) Recorded On Line class 27 Jan | JPR | NEW Video lect | updated 2020 | |
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Webex link in email | Updated 2018 | ||||
Feb 3 | 3 | Light, Matter, and the basics of scatter Recorded 2020 lecture is here. | JPR | Lecture003.ppt | updated 2018 | |
Feb10
| 4 |
| JPR |
| Updated 2018 | |
Feb 17 | 5 | Optical Systems 2019
| JPR | Lecture Video NEW Link to recording 2016 | updated 2016 | |
Feb 24 | 6 |
| JPR | Lecture 6 | Updated 2018 | |
Mar 2 | 7 & 8
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- Detecton Systems 2019 | JPR |
| updated 2018 | |
Mar 9 | 9 & 10 | Fluidic Systems 2019
& | JPR | & | updated 2018 | |
Mar 16 | Spring Break | JPR | Lecture0012.ppt | |||
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Mar23 | On line webex |
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| Updated 2018 | ||
Mar 30 | online webex 12/13 |
| JPR |
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Apr 6 | webex lecture | 2019 Cell Sorting
| JPR | Webex link by email | ||
April 13 | Class Presentations | We will schedule these on webex | ||||
April 20 | Class Presentations | On line webex | ||||
Final Exam | Take home | JPR |
Lecturer: JPR: J. Paul Robinson (contact by email: jpr#flowcyt.cyto.purdue.edu)
Seminars should be 10-15 minutes and must not exceed 20 minutes, you must provide the PPT overview to me at minimum 2 weeks prior to review. I suggest that as soon as you have a topic and some preliminar notes, you discuss this with me so that I can guide you down the best pathway. If I have not reviewed and agreed to your presentation materials, you will automatically lose 50% of the points for this seminar. The whole purpose is to ensure that you present a high quality seminar relevent to the topis, and that your materials are accurate. A copy of your final PPT will be retained and we reserve the right to post this on the web with other seminars if we believe it would be useful to the scientific public. I will not review a seminar with 48 hours of your presentation, unless it has already been given to me for reveiw on at least one previous occasion.
Name | Date | Example Topics (must be related to flow cytometry and cannot be your research project - some examples are below) |
Fluorescent probes for Oxidative Metabolism | ||
NOE | Monoclonal antibodies, Avidin-Biotin technology using Fluorescent Conjugates in Flow cytometry | |
XERC | Principles of Sorting in Flow Cytometry. | |
MSV | Intracellular cytokine determination via flow cytometry | |
MS | Optical Filters used in Flow Cytometry | |
XX | Cell proliferation assessed by flow cytometry using cell tracking dyes | |
Data analysis: How and why? | ||
MDT | Fluidics Systems in Flow Cytometry | |
BK | The principles of spectal overlap | |
GM | Parameters measured in Flow and what they mean | |
HH | Fluorescent probes for apoptosis | |
All materials copyright © J. Paul Robinson 1989-2019 and may not be reproduced for commercial purposes. These slides may be used for educational use only by non-profit institutions and credits must be included for all slides used. In all cases, please request permission for use of these materials. No use is allowed for commercial purposes or for commerical courses of any nature.