BMS 524 Introduction to Confocal Microscopy and Image Analysis -2017

This page is for the use of students who are taking

BMS 524 - 2018 Notes - Vet Path Research Bldg 119

Professor J. Paul Robinson, Purdue University (Email me at jpr(at)


NOTE: The use of these materials is restricted without specific permission of Professor J. Paul Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. While fair use by not-for-profit institutions is allowed, appropriate acknowledgement is requested and should be included on every slide used.



2018 Lecture Schedule


BMS 524 – 15 lectures

Date Lect #Title LectPPT File (Download) View the Lecture
Jan 81The Principles of Microscopy (1) (current download 2018)JPR

30 megs

126 meg (2014)


2016 Lect

2012 lecture

Video lecture (2009)

Jan 17)

2Principles of Microscopy (2) (current download 2018)JPR

3.3 megs

100 meg (2014)


2016 lect

updated 2017
Jan 243Principles of Microscopy (3) (current download 2018)JPR

5.3 megs

112 meg recorded lecture


2016 recorded Lecture

Video lecture (from 2009)

NEW VIDEO Lecture from



4Image Formats and Image Manipulations 2018JPR  2016 lectureUpdated 2018
Jan 245Fluorescence and Fluorescence Probes 2018JPR5.0 megs
updated 2017
Jan  248Components of the microscope 2018JprLecture 8 
Jan 316Novel 3-D microscopy techniques: 2-photon, 4Pi (1)BR updated 2017
Feb 77Novel 3-D microscopy techniques: 2-photon, 4Pi (2)BR12 megsupdated 2017
Feb 148

Principles of 2D digital image analysis - (I) 2018

JPR Updated 2016
Mar 79Principles of 2D digital image analysis (II) 2018JPRLecture 7 
 10Sample Preparation of Confocal MicroscopyJPR2.3 meg PDFUpdated 2017
April 1811

Live Cell Imaging Applications in Confocal Microscopy







Updated 2017





April 2512

Image Presention, Manipulation & Fraud


Updated file

30 megs

Updated 2017

Spectral Imaging Lecture


BR10.6 megs downloadUpdated 2017




You can click on the links to download or directly view the Powerpoint files. NOTE: most of these lectures are relatively large.

JPR (Dr. J. Paul Robinson), BR (Dr. Bartek Rajwa)


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